Using a microRNA panel of circulating exosomes for diagnosis of pancreatic cancer: multicentre case–control study
Using a microRNA panel of circulating exosomes for diagnosis of pancreatic cancer: multicentre case–control study
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使用循环外泌体的 microRNA 组诊断胰腺癌：多中心病例对照研究
DOI：
10.1093/bjs/znac375
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发表时间：
2022
影响因子：
9.6
通讯作者：
Taiping Zhang
中科院分区：
文献类型：
--
作者：
Gang Yang;Jiangdong Qiu;Jianwei Xu;Guangbing Xiong;Fangyu Zhao;Zhe Cao;Guangyu Chen;Yueze Liu;Jinxin Tao;Lianfang Zheng;Lei Wang;Renyi Qin;Taiping Zhang
MethodsThis multicentre study was conducted at three high-volume pancreatic centres in China. A total of 262 peripheral blood samples were obtained from patients with pancreatic cancer, chronic pancreatitis (CP), other pancreatic tumours (OPT), and negative controls (NC; healthy volunteers without chronic or malignant diseases). These were divided into three groups: discovery group (16), preliminary group (56), and large-sample group (190). Exosomal miRNAs were extracted from plasma, and a miRNA microarray was applied to screen the exosomal miRNA expression profiles in the discovery group. MicroRNAs that were differentially expressed between pancreatic cancer and other groups were selected for further validation in the preliminary group and large-sample group by real-time quantitative PCR (RT-qPCR). The exosomal miRNA panel was constructed based on miRNA expression values by logistic regression. The test performance of the panel, CA19-9, and their combination in discriminating pancreatic cancer from CP, NC, and OPT was evaluated using receiver operating characteristic (ROC) curves and areas under the curve (AUCs). Further details of methods are provided in the supplementary material.Results
