Possible role of a peach homolog of UDP-glucose dehydrogenase in fruit development
Possible role of a peach homolog of UDP-glucose dehydrogenase in fruit development
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DOI：
10.1016/j.scienta.2012.11.013
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发表时间：
2013-02
影响因子：
4.3
通讯作者：
Keiko Sato;Emiko Hihara;M. Yamaguchi;K. Kanahama;Y. Kanayama
中科院分区：
文献类型：
--
作者：
Keiko Sato;Emiko Hihara;M. Yamaguchi;K. Kanahama;Y. Kanayama
从商品桃和果实性状不同的日本地方品种果实中提取的RNA进行消减杂交，获得了一个与UDP-葡萄糖脱氢酶(UGD)同源的基因片段，该基因参与了果胶和半纤维素的生物合成，通过UDP-葡萄糖转化为UDP-葡萄糖醛酸。Northern印迹分析证实，该片段在商品品种果实中有较高的表达。从该片段的核苷酸序列中，克隆了包含整个编码区的cDNA，作为UGD的桃树同源物PpUGD1，其推导的氨基酸序列被发现包含对UGD活性重要的保守区。PpUGD1mRNA在未成熟叶片中的表达水平显著高于成熟叶片。桃果实发育过程中PpUGD1mRNA水平的变化与细胞壁物质含量和细胞壁糖酸含量的变化相对应。PpUGD1在商品桃果实中的表达高于日本地方桃品种，PpUGD1在商品桃果实中的表达高于日本地方桃品种。这些发现表明，PpUGD1可能在桃果实发育过程中的细胞壁生物合成中发挥作用。
A cDNA fragment homologous to UDP-glucose dehydrogenase (UGD), which is known to be involved in the biosynthesis of pectin and hemicellulose through the conversion of UDP-glucose to UDP-glucuronic acid, was obtained by subtractive hybridization using RNA extracted from the fruits of the commercial and Japanese native peach cultivars with divergent fruit characteristics. The higher expression of the cDNA fragment in the fruits of the commercial cultivars was confirmed by Northern blot analysis. From the nucleotide sequence of the cDNA fragment, cDNA containing the entire coding region was cloned as a peach homolog of UGD, PpUGD1, and its deduced amino acid sequence was found to contain conserved regions important for UGD activity. The level of PpUGD1 mRNA in immature leaves was much higher than that in mature leaves. A change in the PpUGD1 mRNA level during peach fruit development corresponded to changes in the amount of cell wall material and the cell wall uronic acid content. These were greater in the fruits of the commercial cultivars compared with the Japanese native peach cultivars, and the expression of PpUGD1 was higher in the fruits of the commercial cultivars. These findings suggest a possible role of PpUGD1 in cell wall biosynthesis during peach fruit development.