Assessing the mechanical properties of tissue-mimicking phantoms at different depths as an approach to measure biomechanical gradient of crystalline lens
Assessing the mechanical properties of tissue-mimicking phantoms at different depths as an approach to measure biomechanical gradient of crystalline lens
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DOI：
10.1364/boe.4.002769
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发表时间：
2013-12-01
影响因子：
3.4
通讯作者：
Larin, Kirill V.
中科院分区：
文献类型：
--
作者：
Wang, Shang;Aglyamov, Salavat;Larin, Kirill V.
我们证明了在模拟晶状体皮层和晶核的透明层状幻影样品中，使用样品表面对机械刺激响应的主导频率作为传感弹性特性深度分布的有效指标的可行性。聚焦超声用于无创检测样品表面。利用相敏光学相干层析成像系统以纳米级的灵敏度捕捉表面随时间的动态变化。对样品在超声刺激下的表面响应进行了谱分析，并计算了在特定载荷参数下的主导频率。在均匀和分层组织模拟模型上进行了先导实验。结果表明，不同深度的力学层对试样表面响应的频率影响不同，且与试样的深度依赖弹性有关。研究了超声激发的持续时间和频率对基于光谱的检测的影响。这种非侵入性方法有可能应用于局部和快速评估晶状体力学性能对深度的依赖。(C) 2013年美国光学学会
We demonstrate the feasibility of using the dominant frequency of the sample surface response to a mechanical stimulation as an effective indicator for sensing the depthwise distribution of elastic properties in transparent layered phantom samples simulating the cortex and nucleus of the crystalline lens. Focused ultrasound waves are used to noninvasively interrogate the sample surface. A phase-sensitive optical coherence tomography system is utilized to capture the surface dynamics over time with nanometer scale sensitivity. Spectral analysis is performed on the sample surface response to ultrasound stimulation and the dominant frequency is calculated under particular loading parameters. Pilot experiments were conducted on homogeneous and layered tissue-mimicking phantoms. Results indicate that the mechanical layers located at different depths introduce different frequencies to the sample surface response, which are correlated with the depth-dependent elasticity of the sample. The duration and the frequency of the ultrasound excitation are also investigated for their influences on this spectrum-based detection. This noninvasive method may be potentially applied for localized and rapid assessment of the depth dependence of the mechanical properties of the crystalline lens. (C) 2013 Optical Society of America