The number of unidentified amacrine cells in the mammalian retina.
The number of unidentified amacrine cells in the mammalian retina.
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DOI：
10.1073/pnas.93.25.14906
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发表时间：
1996-12
影响因子：
11.1
通讯作者：
E. Strettoi;R. Masland
中科院分区：
文献类型：
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作者：
E. Strettoi;R. Masland
用荧光探针对兔视网膜中已知的三个最大的无长突细胞群体进行染色，并在一系列视网膜偏心率下进行计数。使用荧光DNA结合分子对视网膜进行复染，并在共聚焦切片中计数内核层中的细胞核总数。根据内核层细胞的总数和无长突细胞所占的已知比例，可以计算出染色群体所占的无长突细胞比例。无长突细胞中，星突细胞占3%，吲哚胺积累细胞占4%，AII细胞占11%。通过将四个较小的无长突细胞群体与吲哚胺积累细胞的数量进行比较，估计它们占所有无长突细胞的4%。因此，兔子视网膜中78%的无长突细胞仅从孤立的例子中得知，如果有的话。这一比例在小鼠、猫和猴的视网膜中相似。很可能存在于中枢神经系统的其他区域中的局部回路神经元的相当大一部分作为群体对于当前技术也是不可见的。
The three largest known populations of amacrine cells in the rabbit retina were stained with fluorescent probes in whole mounts and counted at a series of retinal eccentricities. The retinas were counterstained using a fluorescent DNA-binding molecule and the total number of nuclei in the inner nuclear layer were counted in confocal sections. From the total number of inner nuclear layer cells and the known fraction of them occupied by amacrine cells, the fraction of amacrine cells made up by the stained populations could be calculated. Starburst cells made up 3%, indoleamine-accumulating cells made up 4%, and AII cells made up 11% of all amacrine cells. By referring four smaller populations of amacrine cells to the number of indoleamine-accumulating cells, they were estimated to make up 4% of all amacrine cells. Thus, 78% of all amacrine cells in the rabbit's retina are known only from isolated examples, if at all. This proportion is similar in the retinas of the mouse, cat, and monkey. It is likely that a substantial fraction of the local circuit neurons present in other regions of the central nervous system are also invisible as populations to current techniques.