Improvement of Soybean Agrobacterium-Mediated Transformation Efficiency by Adding Glutamine and Asparagine into the Culture Media.
Improvement of Soybean Agrobacterium-Mediated Transformation Efficiency by Adding Glutamine and Asparagine into the Culture Media.
复制标题
通过在培养基中添加谷氨酰胺和天冬酰胺提高大豆农杆菌介导的转化效率。
DOI：
10.3390/ijms19103039
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发表时间：
2018-10-05
影响因子：
5.6
通讯作者：
Hou W
中科院分区：
文献类型：
--
作者：
Chen L;Cai Y;Liu X;Yao W;Guo C;Sun S;Wu C;Jiang B;Han T;Hou W
转基因大豆作为一种转基因作物，在食品、营养、工业和医药等方面的应用在全球范围内占有最大的份额，高效转化是转基因大豆改良的关键。目前，大豆转化主要采用农杆菌介导法，但与水稻转化效率（90%以上）相比，农杆菌介导法的转化效率仍较低（5%以下）。本文研究了L-谷氨酰胺和/或L-天冬酰胺对农杆菌介导的大豆遗传转化的影响，并探讨了它们在农杆菌介导的大豆遗传转化过程中的可能作用。结果表明，在培养基中单独或同时添加谷氨酰胺和天冬酰胺，可提高不定芽诱导率、伸长率和转化率。谷氨酰胺和天冬酰胺联合应用效果优于单独应用。50 mg/L的L-谷氨酰胺和50 mg/L的L-天冬酰胺可以通过降低GmPRs（GmPR 1、GmPR 4、GmPR 5和GmPR 10）的表达水平和抑制植物的防御反应来提高大豆的转化频率。转基因成功地传递到T1代。本研究为大豆基因工程提供了有益的参考。
As a genetically modified crop, transgenic soybean occupies the largest global scale with its food, nutritional, industrial, and pharmaceutical uses.Efficient transformation is a key factor for the improvement of genetically modified soybean. At present, the Agrobacterium-mediated method is primarily used for soybean transformation, but the efficiency of this method is still relatively low (below 5%) compared with rice (above 90%). In this study, we examined the influence of l-glutamine and/or l-asparagine on Agrobacterium-mediated transformation in soybean and explored the probable role in the process of Agrobacterium-mediated transformation. The results showed that when the amino acids l-glutamine and l-asparagine were added separately or together to the culture medium, the shoot induction frequency, elongation rate, and transformation frequency were improved. The combined effects of l-glutamine and l-asparagine were better than those of l-glutamine and l-asparagine alone. The 50 mg/L l-glutamine and 50 mg/L l-asparagine together can enhance the transformation frequency of soybean by attenuating the expression level of GmPRs (GmPR1, GmPR4, GmPR5, and GmPR10) and suppression of the plant defense response. The transgene was successfully transmitted to the T1 generation. This study will be useful in genetic engineering of soybean.