The Electricidal Effect Is Active in an Experimental Model of Staphylococcus epidermidis Chronic Foreign Body Osteomyelitis
The Electricidal Effect Is Active in an Experimental Model of Staphylococcus epidermidis Chronic Foreign Body Osteomyelitis
复制标题
DOI：
10.1128/aac.00432-09
复制
发表时间：
2009-10-01
影响因子：
4.9
通讯作者：
Patel, Robin
中科院分区：
文献类型：
--
作者：
Del Pozo, Jose L.;Rouse, Mark S.;Patel, Robin
在兔葡萄球菌性慢性异物骨髓炎模型上，比较了小电流(200 MA)电流治疗与静脉注射多西环素治疗的效果，以确定电杀灭作用在体内是否有效。将一个不锈钢种植体和10(4)个浮游表皮葡萄球菌CFU植入胫骨髓腔内。四周后，兔子被分配到三组中的一组，进行为期21天的治疗。对照组(n=10)、强力霉素组(n=14)和电流组(n=15)。多西环素按体重8 mg/kg，每日3次，连续给药。治疗结束后，处死动物，定量培养胫骨。与对照组(中位数4.16[范围3.70至5.66]log(10)cfu/g骨)相比，多西环素组和电流组的细菌负荷显著降低(中位数，2.55[范围，0.5至6.13]log(10)cfu/g骨)和电流组(中位数，1.09[范围，0.50至2.99]log(10)cfu/g骨)(P<；0.0001)。此外，电流治疗的疗效明显优于强力霉素治疗(P=0.035)。电杀灭作用(小电流对细菌生物膜的杀菌活性)在实验性表皮葡萄球菌慢性异物骨髓炎的体内治疗中是活跃的。
Treatment with low-amperage (200 mu A) electrical current was compared to intravenous doxycycline treatment or no treatment in a rabbit model of Staphylococcus epidermidis chronic foreign body osteomyelitis to determine if the electricidal effect is active in vivo. A stainless steel implant and 10(4) CFU of planktonic S. epidermidis were placed into the medullary cavity of the tibia. Four weeks later, rabbits were assigned to one of three groups with treatment administered for 21 days. The groups included those receiving no treatment (n = 10), intravenous doxycycline ( n = 14; 8 mg/kg of body weight three times per day), and electrical current ( n = 15; 200 mu A continuous delivery). Following treatment, rabbits were sacrificed and the tibias quantitatively cultured. Bacterial load was significantly reduced in the doxycycline ( median, 2.55 [ range, 0.50 to 6.13] log(10) CFU/g of bone) and electrical-current ( median, 1.09 [ range, 0.50 to 2.99] log(10) CFU/g of bone) groups, compared to the level for the control group ( median, 4.16 [ range, 3.70 to 5.66] log(10) CFU/g of bone) (P < 0.0001). Moreover, treatment with electrical current was statistically significantly more efficacious (P = 0.035) than doxycycline treatment. The electricidal effect ( the bactericidal activity of low-amperage electrical current against bacterial biofilms) is active in vivo in the treatment of experimental S. epidermidis chronic foreign body osteomyelitis.