Dynein-Mediated Regional Cell Division Reorientation Shapes a Tailbud Embryo
Dynein-Mediated Regional Cell Division Reorientation Shapes a Tailbud Embryo
复制标题
动力蛋白介导的区域细胞分裂重新定向塑造尾芽胚胎
DOI：
10.1016/j.isci.2020.100964
复制
发表时间：
2020
期刊：
影响因子：
5.8
通讯作者：
Gaku Kumano
中科院分区：
文献类型：
--
作者：
Ayaki Nakamoto;Gaku Kumano
细胞分裂方向的调节控制着发育过程中细胞的空间分布，并且对于单向组织转化（如伸长）至关重要。然而，很少有人知道它是否在其他类型的组织形态发生中发挥作用。使用海鞘Halocynthia roretzi，我们发现，不同方向的细胞分裂在未来的躯干（前部）和尾巴（后部）区域的表皮创建一个沙漏状的上皮弯曲的两个区域之间的形状的尾芽胚胎。我们的研究结果表明，后表皮细胞与动力蛋白蛋白的前定位极化，进行动力蛋白依赖的纺锤体旋转，并沿沿着前后轴分裂。这种细胞分裂促进了仅在后部区域中围绕胚胎圆周的收缩和上皮弯曲形成。因此，我们的研究结果提供了一个重要的洞察定向细胞分裂的组织形态发生中的作用。
Regulation of cell division orientation controls the spatial distribution of cells during development and is essential for one-directional tissue transformation, such as elongation. However, little is known about whether it plays a role in other types of tissue morphogenesis. Using an ascidianHalocynthia roretzi, we found that differently oriented cell divisions in the epidermis of the future trunk (anterior) and tail (posterior) regions create an hourglass-like epithelial bending between the two regions to shape the tailbud embryo. Our results show that posterior epidermal cells are polarized with dynein protein anteriorly localized, undergo dynein-dependent spindle rotation, and divide along the anteroposterior axis. This cell division facilitates constriction around the embryo's circumference only in the posterior region and epithelial bending formation. Our findings, therefore, provide an important insight into the role of oriented cell division in tissue morphogenesis.