An ATP-activated, ligand-gated ion channel on a cholinergic presynaptic nerve terminal
An ATP-activated, ligand-gated ion channel on a cholinergic presynaptic nerve terminal
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DOI：
10.1073/pnas.93.5.1859
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发表时间：
1996-03-05
影响因子：
11.1
通讯作者：
Stanley, EF
中科院分区：
文献类型：
--
作者：
Sun, XP;Stanley, EF
ATP最近被确定为中枢和外周神经系统中的快速神经递质。一些研究表明，ATP也可以影响经典神经递质的释放，包括与其共同释放的乙酰胆碱。我们用鸡睫状神经节的杯状突触寻找胆碱能突触前神经末梢上的ATP受体，在电压钳下将ATP脉冲到末梢上，诱发一个短潜伏期的阳离子电流，表现出内向整流和明显的脱敏作用。这种电流在腺苷中没有观察到，但被几种空间限制性ATP类似物模拟，并被苏拉明阻断。ATP激活的单离子通道表现出突出的闪烁，并有约17 pS的电导。我们的研究结果表明，配体门控的P-2X样嘌呤受体的胆碱能突触前神经末梢。
ATP has recently been identified as a fast neurotransmitter in both the central and peripheral nervous systems. Several studies have suggested that ATP can also affect the release of classical neurotransmitters, including acetylcholine with which it is co-released. We have searched for ATP receptors on a cholinergic presynaptic nerve terminal using the calyx-type synapse of the chicken ciliary ganglion, ATP was pulsed onto the terminals under voltage clamp and induced a short latency cation current that exhibited inward rectification and marked desensitization. This current was not seen with adenosine but was mimicked by several sterically restricted ATP analogs and was blocked by suramin. ATP-activated single ion channels exhibited prominent flickering and had a conductance of approximate to 17 pS. Our results demonstrate a ligand-gated P-2X-like purinergic receptor on a cholinergic presynaptic nerve terminal.