In vitro fermentation of potential prebiotic flours from natural sources: Impact on the human colonic microbiota and metabolome
In vitro fermentation of potential prebiotic flours from natural sources: Impact on the human colonic microbiota and metabolome
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DOI：
10.1002/mnfr.201200046
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发表时间：
2012-08-01
影响因子：
5.2
通讯作者：
Costabile, Adele
中科院分区：
文献类型：
--
作者：
Maccaferri, Simone;Klinder, Annett;Costabile, Adele
范围纤维和益生元代表了调节人类肠道微生物组的有用饮食方法。因此，本研究的目的是调查四种面粉（全麦黑麦，全麦小麦，鹰嘴豆和扁豆50：50，大麦研磨谷物）的影响，其特征在于天然高含量的膳食纤维，对肠道微生物群组成和代谢组学输出。方法和结果使用模拟人类结肠的经验证的三阶段连续发酵系统来模拟肠道微生物群的复杂性和多样性。荧光原位杂交用于评估面粉对微生物群组成的影响，而小分子代谢组通过NMR分析和多变量模式识别技术进行评估。用HT29细胞生长曲线法检测细菌代谢产物对肠上皮细胞生长的调节作用。所有四种面粉都显示出对微生物群组成和代谢活性的积极调节。此外，没有一种面粉影响代谢产物对HT29细胞的生长调节潜力。结论我们的研究结果支持利用测试成分开发各种潜在的益生元食品，旨在改善胃肠道健康。
Scope Fibers and prebiotics represent a useful dietary approach for modulating the human gut microbiome. Therefore, aim of the present study was to investigate the impact of four flours (wholegrain rye, wholegrain wheat, chickpeas and lentils 50:50, and barley milled grains), characterized by a naturally high content in dietary fibers, on the intestinal microbiota composition and metabolomic output. Methods and results A validated three-stage continuous fermentative system simulating the human colon was used to resemble the complexity and diversity of the intestinal microbiota. Fluorescence in situ hybridization was used to evaluate the impact of the flours on the composition of the microbiota, while small-molecule metabolome was assessed by NMR analysis followed by multivariate pattern recognition techniques. HT29 cell-growth curve assay was used to evaluate the modulatory properties of the bacterial metabolites on the growth of intestinal epithelial cells. All the four flours showed positive modulations of the microbiota composition and metabolic activity. Furthermore, none of the flours influenced the growth-modulatory potential of the metabolites toward HT29 cells. Conclusion Our findings support the utilization of the tested ingredients in the development of a variety of potentially prebiotic food products aimed at improving gastrointestinal health.