Evidence for in-vivo transfer of mecA DNA between strains of Staphylococcus aureus
Evidence for in-vivo transfer of mecA DNA between strains of Staphylococcus aureus
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DOI：
10.1016/s0140-6736(00)04832-7
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发表时间：
2001-05-26
期刊：
影响因子：
168.9
通讯作者：
Fluit, AC
中科院分区：
文献类型：
--
作者：
Wielders, CLC;Vriens, MR;Fluit, AC
金黄色葡萄球菌被认为是通过水平转移获得了mecA DNA。限制性内切核酸酶切割DNA的特定序列，从而形成DNA指纹，用于比较细菌之间的完整基因组或特定基因。我们从一名从未接触过MRSA的新生儿中分离出一种流行的mecA（-）甲氧西林敏感金黄色葡萄球菌基因型，随后又分离出一种罕见的等基因型mecA（+）耐甲氧西林金黄色葡萄球菌（MRSA）。该MRSA含有mecA DNA，与从该患者分离的凝固酶阴性葡萄球菌菌株相同，但与其他MRSA基因型不同。我们认为这种MRSA是通过两种葡萄球菌之间的mecA DNA水平转移在体内形成的。
Staphylococcus aureus is thought to have acquired mecA DNA by horizontal transfer. DNA fingerprints made by restriction nucleases that cut certain sequences of DNA are used to compare complete genomes or particular genes between bacteria. We isolated an epidemic mecA(-) meticillin-susceptible S aureus genotype and, subsequently, a rare isogeneic mecA(+) meticillin-resistant S aureus (MRSA) genotype from a neonate who had never been in contact with MRSA. This MRSA contained mecA DNA that was identical to that in a coagulase-negative staphylococcal strain isolated from this patient, but different from other MRSA genotypes. We believe that this MRSA was formed in vivo by horizontal transfer of the mecA DNA between two staphylococcal species.