Greenbug (Schizaphis graminum) herbivory significantly impacts protein and phosphorylation abundance in switchgrass (Panicum virgatum)
Greenbug (Schizaphis graminum) herbivory significantly impacts protein and phosphorylation abundance in switchgrass (Panicum virgatum)
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DOI：
10.1038/s41598-020-71828-8
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发表时间：
2020-09
影响因子：
4.6
通讯作者：
Prince Zogli;S. Alvarez;M. Naldrett;N. Palmer;K. Koch;Lise Pingault;J. Bradshaw;P. Twigg;T. Heng-moss;J. Louis;G. Sarath
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文献类型：
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作者：
Prince Zogli;S. Alvarez;M. Naldrett;N. Palmer;K. Koch;Lise Pingault;J. Bradshaw;P. Twigg;T. Heng-moss;J. Louis;G. Sarath
柳枝稷是生物燃料生产的重要作物，但它也是绿蝽（Schizaphis graminumRondani; GB）的宿主。虽然转录组学研究已经做了推断的分子机制，对GB的入侵对柳枝稷蛋白表达和磷酸化调控的影响知之甚少。柳枝稷栽培品种夏季蛋白质组和磷酸化蛋白质组的全球响应监测无标记蛋白质组学鸟枪在GB感染和未感染的对照植物在感染后10天。肽匹配总共3，594蛋白质进行了鉴定，429是差异表达的蛋白质在GB-侵染的植物相对于未侵染的对照植物。其中，291个和138个分别被GB侵染上调和下调。磷酸化蛋白质组分析从350个磷酸化肽中鉴定出310个差异磷酸化蛋白质（DP），共399个磷酸化位点。与苏氨酸磷酸化位点（21%）相比，这些磷酸肽具有更多的丝氨酸磷酸化残基（79%）。总体而言，KEGG途径分析表明，GB喂养导致富集的蛋白质的生物合成的植物防御次生代谢产物的重要和抑制的蛋白质参与光合作用的积累。有趣的是，防御调节剂，如萜烯合酶，木瓜蛋白酶样半胱氨酸蛋白酶，丝氨酸羧肽酶，脂氧合酶2在蛋白质组水平上调，证实了先前发表的转录组学数据。
Switchgrass (Panicum virgatumL.) is an important crop for biofuel production but it also serves as host for greenbugs (Schizaphis graminumRondani; GB). Although transcriptomic studies have been done to infer the molecular mechanisms of plant defense against GB, little is known about the effect of GB infestation on the switchgrass protein expression and phosphorylation regulation. The global response of the switchgrass cultivar Summer proteome and phosphoproteome was monitored by label-free proteomics shotgun in GB-infested and uninfested control plants at 10 days post infestation. Peptides matching a total of 3,594 proteins were identified and 429 were differentially expressed proteins in GB-infested plants relative to uninfested control plants. Among these, 291 and 138 were up and downregulated by GB infestation, respectively. Phosphoproteome analysis identified 310 differentially phosphorylated proteins (DP) from 350 phosphopeptides with a total of 399 phosphorylated sites. These phosphopeptides had more serine phosphorylated residues (79%), compared to threonine phosphorylated sites (21%). Overall, KEGG pathway analysis revealed that GB feeding led to the enriched accumulation of proteins important for biosynthesis of plant defense secondary metabolites and repressed the accumulation of proteins involved in photosynthesis. Interestingly, defense modulators such as terpene synthase, papain-like cysteine protease, serine carboxypeptidase, and lipoxygenase2 were upregulated at the proteome level, corroborating previously published transcriptomic data.