Growth kinetics rather than stress accelerate telomere shortening in cultures of human diploid fibroblasts in oxidative stress-induced premature senescence
Growth kinetics rather than stress accelerate telomere shortening in cultures of human diploid fibroblasts in oxidative stress-induced premature senescence
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DOI：
10.1016/s0014-5793(01)02679-5
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发表时间：
2001-08-03
期刊：
影响因子：
3.5
通讯作者：
Toussaint, O
中科院分区：
文献类型：
--
作者：
Dumont, P;Royer, V;Toussaint, O
WI-38人二倍体成纤维细胞在暴露于四种亚细胞毒性的100 μ M叔丁基过氧化氢（t-BHP）应激后经历加速的端粒缩短（490 bp/应激）和生长停滞，其中每两个群体应激（PD）时应激。在亚细胞毒性的160 μ M H2 O2应激或5次重复的30 μ M t-BHP应激沿着相同的PD后，仅在应激后的第一次PD期间分别观察到322 +/- 55和380 +/- 129 bp的端粒缩短。在应激后恢复增殖的细胞的百分比表明，这种端粒缩短是由于在应激后的第一次PD期间达到汇合所完成的细胞分裂的数量。(C)2001年欧洲生物化学学会联合会。由Elsevier Science B. V.出版，版权所有。
WI-38 human diploid fibroblasts underwent accelerated telomere shortening (490 bp/stress) and growth arrest after exposure to four subcytotoxic 100 muM tert-butylhydroperoxide (t-BHP) stresses, with a stress at every two population doublings; (PD). After subcytotoxic 160 muM H2O2 stress or five repeated 30 muM t-BHP stresses along the same PD, respectively a 322 +/- 55 and 380 +/- 129 bp telomere shortening was observed only during the first PD after stress. The percentage of cells resuming proliferation after stress suggests this telomere shortening is due to the number of cell divisions accomplished to reach confluence during the first PD after stress. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.