Chronic idiopathic neutropenia associated with abnormal expression of granulocyte colony-stimulating factor mRNA of bone marrow stromal cells.
Chronic idiopathic neutropenia associated with abnormal expression of granulocyte colony-stimulating factor mRNA of bone marrow stromal cells.
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慢性特发性中性粒细胞减少症与骨髓基质细胞粒细胞集落刺激因子 mRNA 表达异常相关。
DOI：
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发表时间：
1994
影响因子：
2.1
通讯作者：
Y. Niitsu
中科院分区：
文献类型：
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作者：
Y. Kohgo;Y. Hirayama;T. Matsunaga;J. Kato;S. Sakamaki;Y. Niitsu
慢性特发性中性粒细胞减少症是一种不常见的疾病，其特征是中性粒细胞水平低，没有任何病因。我们报告的细节4例间质细胞因子mRNA的表达进行了研究，通过逆转录聚合酶链反应。3例患者骨髓细胞粒细胞集落刺激因子（G-CSF）mRNA表达水平较低，且骨髓粒细胞-巨噬细胞集落形成单位（CFU-GM）正常。1例患者G-CSF mRNA表达增加，CFU-GM表达减少。未观察到其他细胞因子包括白细胞介素（IL）-1 β、IL-6和IL-8的异常表达。这些发现表明，基质细胞的细胞因子mRNA分析是有用的病因的阐明。
Chronic idiopathic neutropenia is an uncommon condition characterized by a low level of neutrophils without any causative disease. We report the details of 4 patients whose stromal cytokine mRNA expressions were examined by reverse transcription polymerase chain reaction. Three patients showed a lower expression of granulocyte colony-stimulating factor (G-CSF) mRNA both in constructive and lipopolysaccharide (LPS)-induced conditions and normal colony forming unit-granulocyte-macrophage (CFU-GM) from the bone marrow cells. One patient showed an increase of G-CSF mRNA and a decrease of CFU-GM. No abnormal expression of other cytokines including interleukin (IL)-1 beta, IL-6 and IL-8 were observed. These findings indicate that cytokine mRNA analysis of stromal cells is useful for elucidation of the etiology.