Possible involvement of a tetrahydrobiopterin in photoreception for UV-B-induced anthocyanin synthesis in carrot.
Possible involvement of a tetrahydrobiopterin in photoreception for UV-B-induced anthocyanin synthesis in carrot.
复制标题
四氢生物蝶呤可能参与了胡萝卜中 UV-B 诱导的花青素合成的光感受。
DOI：
10.1111/php.12302
复制
发表时间：
2014
影响因子：
3.3
通讯作者：
Iseki M. and Watanabe M.
中科院分区：
文献类型：
--
作者：
Takeda J.;Nakata R.;Ueno H.;Murakami A.;Iseki M. and Watanabe M.
我们以前的研究表明，在培养的胡萝卜细胞中，UV-B诱导的花青素苷积累的作用光谱表明，一种还原形式的蝶呤，可能是四氢生物蝶呤，有助于UV-B光感受。在这份报告中，我们提供了更多的证据参与的蝶呤在紫外B光传感。UV B诱导的苯丙氨酸解氨酶（PAL）活性被N-乙酰血清素（四氢生物蝶呤生物合成的抑制剂）显著抑制，并且这种抑制通过加入生物蝶呤或四氢生物蝶呤而部分恢复。此外，在N-乙酰5-羟色胺处理的细胞中，通过放射性标记实验检测到与生物蝶呤特异性结合的蛋白质。此外，一种有效的电子转移抑制剂diphenyleniodonium完全抑制UV-B-诱导的PAL活性。这些结果表明，胡萝卜细胞中存在一种未鉴定的UV-B光感受器（UVR 8除外，UVR 8是最初在拟南芥中发现的基于色氨酸的UV-B传感器），蝶呤减少。在重新检查已发表的作用光谱后，我们认为花青素的合成是由这两个UV B传感器协调调节的。
Our previous studies of action spectra for UV‐B‐induced anthocyanin accumulation in cultured carrot cells indicated that a reduced form of pterin, possibly tetrahydrobiopterin, contributes to UV‐B photoreception. In this report, we provide additional evidence for the involvement of pterin in UV‐B light sensing. UV‐B‐induced phenylalanine ammonia‐lyase (PAL) activity was considerably suppressed by N‐acetylserotonin (an inhibitor of tetrahydrobiopterin biosynthesis), and this suppression was partially recovered by adding biopterin or tetrahydrobiobiopterin. In addition, protein(s) specifically bound to biopterin were detected by radiolabeling experiments in N‐acetylserotonin‐treated cells. Furthermore, diphenyleneiodonium, a potent inhibitor of electron transfer, completely suppressed UV‐B‐induced PAL activity. These results suggest the occurrence of an unidentified UV‐B photoreceptor (other than UVR8, the tryptophan‐based UV‐B sensor originally identified inArabidopsis) with reduced pterin in carrot cells. After reexamining published action spectra, we suggest that anthocyanin synthesis is coordinately regulated by these two UV‐B sensors.