The inhibitory effect of tachyplesin I on thrombosis and its mechanisms
The inhibitory effect of tachyplesin I on thrombosis and its mechanisms
复制标题
鲎素I抑制血栓形成的作用及其机制
DOI：
10.1111/cbdd.13570
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发表时间：
2019-06
影响因子：
3
通讯作者：
Zhang Haitao
中科院分区：
文献类型：
--
作者：
Li Huimin;Liu Bin;Wu Jun;Yu Huajun;Huang Hui;Chen Xi;Chen Baoan;Wu Shang;Ma Jingyao;Liu Wen;Chen Xiaoyi;Lan Liubo;He Zhan;Zhang Haitao
血栓性疾病是心血管疾病的主要病因。本研究旨在探讨鲎素I对血小板聚集和血栓形成的影响。用全血聚集计分析血小板聚集。采用小鼠研究鲎素I对体内血栓形成的影响。Tachyplesin I以剂量依赖性方式抑制凝血酶诱导的血小板聚集。此外，鲎素I通过腹膜内注射（0.1、0.2或0.4 mg/kg）或灌胃给药（15、30或60 mg/kg）显著减少角叉菜胶诱导的尾部血栓形成模型中的血栓形成。Tachyplesin I还延长出血时间（BT）和凝血时间（CT）。结果表明，鲎素I通过干扰PI 3 K/AKT通路抑制血小板聚集和血栓形成。Tachyplesin I在300 mg/kg剂量下静脉注射对小鼠无明显毒性。结果表明，鲎素I抑制血栓形成，毒性低。表明鲎素I具有开发新的抗血栓药物的潜力。
Thrombotic diseases are major cause of cardiovascular diseases. This study was designed to investigate the effect of tachyplesin I on platelet aggregation and thrombosis. Platelet aggregation was analysed with a whole blood aggregometer. The mice were employed to investigate the effect of tachyplesin I on thrombosis in vivo. Tachyplesin I inhibited thrombin‐induced platelet aggregation in a dose‐dependent manner. Furthermore, tachyplesin I significantly reduced thrombosis in carrageenan‐induced tail thrombosis model by intraperitoneal injection (0.1, 0.2 or 0.4 mg/kg) or intragastric administration (15, 30 or 60 mg/kg). Tachyplesin I also prolonged the bleeding time (BT) and clotting time (CT). The results revealed that tachyplesin I inhibited platelet aggregation and thrombosis by interfering the PI3K/AKT pathway. Tachyplesin I did not show significantly toxicity to mice under 300 mg/kg via intravenous injection. The results show that tachyplesin I inhibits thrombosis and has low toxicity. It is suggested that tachyplesin I has the potential to develop a new anti‐thrombotic drug.
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影响因子：
1.4
作者：
Qian Liu;Da-sheng Dang;Yu-feng Chen;Ming Yan;Guo-bing Shi;Qingchun Zhao
通讯作者：
Qian Liu;Da-sheng Dang;Yu-feng Chen;Ming Yan;Guo-bing Shi;Qingchun Zhao
影响因子：
13.3
作者：
Gyori, David;Csete, Daniel;Benko, Szilvia;Kulkarni, Suhasini;Mandl, Peter;Dobo-Nagy, Csaba;Vanhaesebroeck, Bart;Stephens, Len;Hawkins, Phillip T.;Mocsai, Attila
通讯作者：
Mocsai, Attila
影响因子：
3.3
作者：
Jing Xiong;Weirong Fang;Weirong Fang;L. Bai;Jianli Huo;Yi Kong;Yunman Li
通讯作者：
Jing Xiong;Weirong Fang;Weirong Fang;L. Bai;Jianli Huo;Yi Kong;Yunman Li
影响因子：
3.1
作者：
Fareed J;Adiguzel C;Thethi I
通讯作者：
Thethi I
影响因子：
2.7
作者：
S. Iwanaga;S. Kawabata;T. Muta
通讯作者：
S. Iwanaga;S. Kawabata;T. Muta