Microtubule-mediated transport of incoming herpes simplex virus 1 capsids to the nucleus.
Microtubule-mediated transport of incoming herpes simplex virus 1 capsids to the nucleus.
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DOI：
10.1083/jcb.136.5.1007
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发表时间：
1997-03-10
期刊：
影响因子：
--
通讯作者：
Helenius A
中科院分区：
文献类型：
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作者：
Sodeik B;Ebersold MW;Helenius A
单纯疱疹病毒1型与宿主细胞的质膜融合，并且进入的衣壳有效且快速地穿过细胞质转运到核孔复合物，在那里病毒DNA基因组被释放到核质中。使用生物化学测定，免疫荧光和免疫电镜在存在和不存在的微管解聚剂，它表明，在Vero细胞的胞质衣壳运输是由微管介导的。抗体标记揭示了附着的动力蛋白，负端定向，微管依赖性电机，病毒衣壳。我们提出，进入的衣壳与微管结合，并利用动力蛋白将它们从细胞外围推动到细胞核。
Herpes simplex virus 1 fuses with the plasma membrane of a host cell, and the incoming capsids are efficiently and rapidly transported across the cytosol to the nuclear pore complexes, where the viral DNA genomes are released into the nucleoplasm. Using biochemical assays, immunofluorescence, and immunoelectron microscopy in the presence and absence of microtubule depolymerizing agents, it was shown that the cytosolic capsid transport in Vero cells was mediated by microtubules. Antibody labeling revealed the attachment of dynein, a minus end–directed, microtubule-dependent motor, to the viral capsids. We propose that the incoming capsids bind to microtubules and use dynein to propel them from the cell periphery to the nucleus.