Overexpression of LAMC1 predicts poor prognosis and enhances tumor cell invasion and migration in hepatocellular carcinoma.
Overexpression of LAMC1 predicts poor prognosis and enhances tumor cell invasion and migration in hepatocellular carcinoma.
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LAMC1的过度表达可预测肝细胞癌的不良预后并增强肿瘤细胞的侵袭和迁移
DOI：
10.7150/jca.21038
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发表时间：
2017
影响因子：
3.9
通讯作者：
Chen M
中科院分区：
文献类型：
--
作者：
Zhang Y;Xi S;Chen J;Zhou D;Gao H;Zhou Z;Xu L;Chen M
LAMC 1编码一种细胞外基质蛋白--层粘连蛋白γ1链，参与组织发育、肿瘤细胞侵袭和转移等多种生物学和病理学过程。在本研究中，我们证明了LAMC 1蛋白和mRNA水平升高，肝癌组织样本相比，非癌组织样本，根据蛋白质印迹分析，免疫组化（IHC）和芯片。此外，LAMC 1高表达与包膜不完全（p=0.014）、总体（OS，p=0.02）和无病生存期（DFS，p=0.014）呈正相关。利用细胞系，我们证明，LAMC 1的mRNA和蛋白质的水平显着高于在肝癌细胞系比在LO 2细胞系。利用siRNA技术抑制LAMC 1的表达后，细胞增殖、迁移和侵袭能力明显降低。总之，这些数据表明LAMC 1在HCC中富集; LAMC 1的过表达预测不良预后，并增强肿瘤细胞侵袭和迁移。LAMC 1可能是一个新的预测肝癌预后的生物标志物，也可能是一个有用的治疗靶点。
LAMC1 encodes an extracellular matrix protein, laminin γ1 chain, which is involved in several biological and pathological processes including tissue development, tumor cell invasion and metastasis. In present study, we demonstrated that both LAMC1 protein and mRNA levels were elevated in HCC tissue samples compared with non-cancerous tissue samples according to western blot analyses, immunohistochemistry (IHC) and microarray. Moreover, high LAMC1 expression was positively correlated with incomplete encapsulation (p=0.014), poor overall (OS, p=0.02) and disease-free survival (DFS, p=0.014). Using cell lines, we demonstrated that the levels of LAMC1 mRNA and protein were significantly higher in HCC cell lines than that in LO2 cell line. After the expression of LAMC1 was depressed by siRNA technique, the cell proliferation, migration and invasion were depressed significantly. Taken together, these data suggest that LAMC1 is enriched in HCC; overexpression of LAMC1 predicts poor prognosis, and enhances tumor cell invasion and migration. LAMC1 might be a new biomarker predictive of HCC prognosis and might also be a useful treatment target.
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影响因子：
--
作者：
Arita T;Ichikawa D;Konishi H;Komatsu S;Shiozaki A;Ogino S;Fujita Y;Hiramoto H;Hamada J;Shoda K;Kosuga T;Fujiwara H;Okamoto K;Otsuji E
通讯作者：
Otsuji E
细胞表面层粘连蛋白锚定的丧失会促进肿瘤生长，并与不良的临床结果相关。
影响因子：
11.2
作者：
Akhavan A;Griffith OL;Soroceanu L;Leonoudakis D;Luciani-Torres MG;Daemen A;Gray JW;Muschler JL
通讯作者：
Muschler JL
影响因子：
4.7
作者：
Kong, Lu;Schaefer, Georg;Klocker, Helmut
通讯作者：
Klocker, Helmut
影响因子：
8.4
作者：
通讯作者：
--
TACC3 促进干性，是肝细胞癌的潜在治疗靶点。
影响因子：
--
作者：
Zhou DS;Wang HB;Zhou ZG;Zhang YJ;Zhong Q;Xu L;Huang YH;Yeung SC;Chen MS;Zeng MS
通讯作者：
Zeng MS