RING finger palmitoylation of the endoplasmic reticulum Gp78 E3 ubiquitin ligase
RING finger palmitoylation of the endoplasmic reticulum Gp78 E3 ubiquitin ligase
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DOI：
10.1016/j.febslet.2012.06.011
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发表时间：
2012-07-30
期刊：
影响因子：
3.5
通讯作者：
Nabi, Ivan R.
中科院分区：
文献类型：
--
作者：
Fairbank, Maria;Huang, Kun;Nabi, Ivan R.
Gp78是内质网相关降解途径中的E3泛素连接酶。我们发现flag标记的gp78在环指基元内经历了巯基半胱氨酸棕榈酰化（s -棕榈酰化），负责其泛素连接酶活性。筛选19个棕榈酰酰基转移酶（PATs），鉴定出5个增加gp78环指棕榈酰化的酶。内质网Myc-DHHC6的过表达促进了gp78外周内质网的分布，而RING finger突变和棕榈酰化抑制剂2-溴铝酸盐限制了gp78在中央内质网的分布。因此，环指半胱氨酸的棕榈酰化调节gp78向外周内质网的分布。(c) 2012年欧洲生化学会联合会。Elsevier B.V.版权所有。
Gp78 is an E3 ubiquitin ligase within the endoplasmic reticulum-associated degradation pathway. We show that Flag-tagged gp78 undergoes sulfhydryl cysteine palmitoylation (S-palmitoylation) within the RING finger motif, responsible for its ubiquitin ligase activity. Screening of 19 palmitoyl acyl transferases (PATs) identified five that increased gp78 RING finger palmitoylation. Endoplasmic reticulum (ER)-localized Myc-DHHC6 overexpression promoted the peripheral ER distribution of Flag-gp78 while RING finger mutation and the palmitoylation inhibitor 2-bromopalmitate restricted gp78 to the central ER. Palmitoylation of RING finger cysteines therefore regulates gp78 distribution to the peripheral ER. (c) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.