PARP-1 genetic polymorphism associated with radiation sensitivity of non-small cell lung cancer.
PARP-1 genetic polymorphism associated with radiation sensitivity of non-small cell lung cancer.
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DOI：
10.3389/pore.2022.1610751
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发表时间：
2022
影响因子：
2.8
通讯作者：
Guo, Genyan
中科院分区：
文献类型：
--
作者：
Wang, Hetong;Xie, Haitao;Wang, Shuying;Zhao, Jiaying;Gao, Ya;Chen, Jun;Zhao, Yuxia;Guo, Genyan
约70%的非小细胞肺癌(NSCLC)患者需要放疗。然而，由于辐射敏感性的不同，对于相同的病理和治疗选择，治疗结果可能不同。聚(ADP-核糖)聚合酶1(PARP-1)是DNA修复的关键基因，参与碱基切除修复以及电离辐射和氧化损伤引起的单链断裂修复。为探讨PARP-1基因多态性与非小细胞肺癌放射敏感性的关系，我们收集了141例接受三维适形放射治疗的非小细胞肺癌患者。测量照射前和照射后大体肿瘤体积(GTV)，计算肿瘤消退率。采用TaqMan实时定量聚合酶链式反应对单核苷酸多态(SNPs)进行基因分型。PARP-1基因C/C、C/G和G/G基因频率分别为14.2%、44.7%和41.1%。放射治疗后肿瘤平均消退率为35.1%±0.192。C/C型中期RT肿瘤消退率为44.6%±0.170，高于C/G型和G/G型(分别为32.4%±0.196和34.8%±0.188)，差异有统计学意义(F=3.169，P=0.045)。C/C基因携带者肿瘤消退率较高，提示G等位基因是放射治疗的保护因素。用肿瘤消退率的中位数34%将整个队列分为两组，发现PARP-1基因rs3219073的频率分布在两组之间有显著差异(p<；0.05)。这些结果表明，PARP-1基因多态性可能影响患者的放射敏感性，预测放射治疗的疗效。因此，这为开发新的治疗靶点以改善放射治疗结果提供了机会。
About 70% of non-small cell lung cancer (NSCLC) patients require radiotherapy. However, due to the difference in radiation sensitivity, the treatment outcome may differ for the same pathology and choice of treatment. Poly (ADP-ribose) polymerase 1 (PARP-1) is a key gene responsible for DNA repair and is involved in base excision repair as well as repair of single strand break induced by ionizing radiation and oxidative damage. In order to investigate the relationship between PARP-1 gene polymorphism and radiation sensitivity in NSCLC, we collected 141 primary NSCLC patients undergoing three-dimensional conformal radiotherapy. For each case, the gross tumor volumes (GTV) before radiation and that after 40 Gy radiation were measured to calculate the tumor regression rate. TaqMan real-time polymerase chain reaction was performed to genotype the single-nucleotide polymorphisms (SNPs). Genotype frequencies for PARP-1 genotypes were 14.2% for C/C, 44.7% for C/G and 41.1% for G/G. The average tumor regression rate after 40 Gy radiation therapy was 35.1% ± 0.192. Tumor regression rate of mid-term RT of C/C genotype was 44.6% ± 0.170, which was higher than that of genotype C/G and G/G (32.4% ± 0.196 and 34.8% ± 0.188, respectively) with statistical significance (F = 3.169 p = 0.045). The higher tumor regression rate in patients with C/C genotype suggested that G allele was a protective factor against radiation therapy. Using the median tumor regression rate of 34%, we divided the entire cohort into two groups, and found that the frequency distribution of PARP-1 gene rs3219073 had significant difference between these two groups (p < 0.05). These results showed that PARP-1 gene polymorphism may affect patient radiation sensitivity and predict the efficacy of radiotherapy. It therefore presents an opportunity for developing new therapeutic targets to improve radiotherapy outcome.
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影响因子：
16
作者：
Krishnakumar R;Kraus WL
通讯作者：
Kraus WL
影响因子：
45.3
作者：
Fong, Peter C.;Yap, Timothy A.;Kaye, Stan B.
通讯作者：
Kaye, Stan B.
影响因子：
7.4
作者：
Goto, Miho;Ueda, Keisuke;Kanazawa, Kazuki
通讯作者：
Kanazawa, Kazuki
DOI：
10.1016/j.ijrobp.2005.11.012
发表时间：
2006-03-15
影响因子：
7
作者：
Ramsey, CR;Langen, KM;Seibert, RM
通讯作者：
Seibert, RM
影响因子：
3.5
作者：
Chalmers, Anthony J.;Lakshman, Mina;Bristow, Robert G.
通讯作者：
Bristow, Robert G.