Induction of liver microsomal cytochrome P-450 and associated monooxygenases by octachlorostyrene in the rat.
Induction of liver microsomal cytochrome P-450 and associated monooxygenases by octachlorostyrene in the rat.
复制标题
八氯苯乙烯诱导大鼠肝微粒体细胞色素 P-450 和相关单加氧酶。
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
E. Dybing
中科院分区：
文献类型：
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作者：
J. Holme;E. Dybing
本文研究了鱼类中存在的氯代烃类污染物八氯苯乙烯(OCS)和六氯苯(HCB)对大鼠细胞色素p-450系统的诱导作用。单次注射50 mg/kg及以上剂量的OCS和HCB可使小鼠的微粒体蛋白a、细胞色素p-450含量、细胞色素p-450还原酶、乙基吗啡N-脱甲基酶、4-硝基苯甲醚O-脱甲基酶、乙酰苯胺4-羟基酶活性增加，但对苯并(A)芘羟基酶活性无明显影响。重复给药14天后，也出现了类似的诱导模式。十二烷基硫酸钠聚丙烯酰胺凝胶电泳法显示，OCS和HCB处理的大鼠的微粒体蛋白质增加，与苯巴比妥(PB)诱导后的增加相似。在本实验条件下，OCS和HCB均为PB型诱导剂。
The inducing effects of octachlorostyrene (OCS) and hexachlorobenzene (HCB), chlorinated hydrocarbon contaminants found in fish, on the microsomal cytochrome p-450 system were studied in rats. Administration of single doses of OCS and HCB at 50mg/kg intraperitoneally and higher led to increases in microsomal protein an cytochrome p-450 content, cytochrome p-450 reductase, ethylmorphine N-demethylase, 4-nitroanisole O-demethylase, acetanilide 4-hydroxylase, but not in benzo(a)pyrene hydroxylase activities. Similar patterns of induction were seen after 14 days repeated intraperitoneal or oral dosing. Siodium dodecyl sulfate polyacrylamide gel electrophoresis of microsomes from OCS and HCB treated rats showed increases in proteins similar to those found after phenobarbital (PB) induction. Under the present experimental conditions OCS and HCB were found to be PB-type inducers.