Absolute Emission Spectra from Bacillus subtilis and Escherichia coli Vegetative Cells in Solution.
Absolute Emission Spectra from Bacillus subtilis and Escherichia coli Vegetative Cells in Solution.
复制标题
溶液中枯草芽孢杆菌和大肠杆菌营养细胞的绝对发射光谱。
作者：
M. Seaver;D. Roselle;J. Pinto;J. Eversole
用波长在270 ~ 300 nm之间的可调谐激光激发，测定了洗涤过的枯草芽孢杆菌和大肠杆菌稀悬浮液的光谱分辨发射（270-560 nm）。积分绝对发射截面随激发波长的减小而增加，范围为1.8 × 10（-12）至6.0 × 10（-11）cm（2）/（粒子sr）。340 nm附近的发射带占主导地位的所有观察到的光谱。在每个激发波长处，光谱分辨的发射来自E. coli和B.枯草杆菌悬浮液是不可区分的。
Spectrally resolved emission (270-560 nm) from dilute suspensions of washed Bacillus subtilis and Escherichia coli were measured by use of tunable laser excitation between 270 and 300 nm. Integrated absolute emission cross sections increase with decreasing excitation wavelength and range from 1.8 x 10(-12) to 6.0 x 10(-11) cm(2)/(particle sr). An emission band near 340 nm dominates all observed spectra. At each excitation wavelength spectrally resolved emissions from the E. coli and B. subtilis suspensions are indistinguishable.