Application of PEG and EGCG modified collagen-base membrane to promote osteoblasts proliferation
Application of PEG and EGCG modified collagen-base membrane to promote osteoblasts proliferation
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应用PEG和EGCG修饰胶原基膜促进成骨细胞增殖
DOI：
10.1016/j.msec.2017.02.157
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发表时间：
2017-07-01
影响因子：
7.9
通讯作者：
Man, Yi
中科院分区：
文献类型：
--
作者：
Chu, Chenyu;Deng, Jia;Man, Yi
胶原膜具有理想的生物学特性，在引导骨再生(GBR)中可作为支持成骨细胞渗透和增殖的屏障。然而，纯胶原缺乏理想的力学性能，还会导致炎症，导致进行性骨吸收。在我们前期的研究中，EGCG交联型胶原膜表现出更好的力学性能和抗炎作用。然而，较高浓度的EGCG可能并不能提高细胞存活率。因此，考虑到聚乙二醇具有更好的生物相容性，我们提出了一种表面修饰聚乙二醇的增强型EGCG交联型胶原膜，以提高细胞存活率和细胞黏附能力。扫描电子显微镜图像显示，PEGEGCG-胶原膜表面纤维聚集更光滑。傅里叶变换红外光谱分析表明，EGCG和聚乙二醇组分的加入保持了聚乙二醇组分的结构特征。细胞计数试剂盒(CCK-8)和活/死实验表明，修饰后的细胞存活率显著提高。免疫组织化学染色显示细胞骨架形态较好。此外，酶联免疫吸附实验显示，聚乙二醇-EGCG-胶原膜显著降低了MG63细胞分泌炎症因子的水平。综上所述，我们的研究结果表明，聚乙二醇EGCG胶原膜在保持细胞结构完整、促进细胞活性和调节促炎细胞因子分泌等方面，有可能应用于GBR。(C)2017爱思唯尔B.V.保留所有权利。
Collagen membranes possess ideal biological properties and can be served as a barrier for supporting infiltration and proliferation of osteoblasts in guided bone regeneration (GBR). However, pure collagen lacks desirable mechanical properties and also leads to inflammation, resulting in progressive bone resorption. In our previous study, EGCG cross-linked collagen membranes exhibit better mechanical properties and anti-inflammatory effect. However, higher concentration of EGCG may not improve cell viability. Herein, we present an enhanced EGCG cross-linked collagen membranes with surface modification of PEG to improve cell viability and cell adhesion, considering the better biocompatibility of PEG. Scanning electron microscope images showed that PEGEGCG-collagen membrane exhibited smoother surface fiber aggregates. Fourier transform infrared spectroscopy demonstrated that the structure characteristics were maintained after addition of EGCG and PEG. Cell viability was significantly increased after modification of PEG, as determined by the Cell Counting Kit-8 (CCK-8) and live/dead assay. Better shapes of cytoskeleton were observed in immunostaining images. Additionally, enzyme-linked immunosorbent assay showed PEG-EGCG-collagen membrane significantly decreased the level of inflammatory factors secreted by MG63 cells. Collectively, with respect to all the aspects including intact structure, cell viability promotion and mediation of pro-inflammatory cytokine secretion, our results indicate that PEG-EGCG-collagen membrane might be used in GBR applications. (C) 2017 Elsevier B.V. All rights reserved.