Structure, recognition and discrimination in RNA aptamer complexes with cofactors, amino acids, drugs and aminoglycoside antibiotics.
Structure, recognition and discrimination in RNA aptamer complexes with cofactors, amino acids, drugs and aminoglycoside antibiotics.
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DOI：
10.1016/s1389-0352(99)00003-3
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发表时间：
2000-03-01
影响因子：
4.1
通讯作者：
Suri, A K
中科院分区：
文献类型：
--
作者：
Patel, D J;Suri, A K
通过使用体外选择技术，一些RNA适配子被筛选出来，因为它们具有高亲和力和高特异性地结合配体的能力。在过去的4年里，已经解决了许多这类络合物的三维溶液结构。本文综述了辅因子FMN和AMP、氨基酸精氨酸和瓜氨酸、药物茶碱和氨基糖苷类抗生素妥布霉素在溶液中结合的RNA适配子的结构特征。分析这些复合体的结构特征，可以识别RNA适配子结构中的分子主题，识别和区分。
Through the use of in vitro selection techniques, a number of RNA aptamers have been selected for their ability to bind ligands with high affinity and specificity. The three-dimensional solution structures of a number of these complexes have been solved within the last 4 years. This review focuses on the structural characterization of the RNA aptamers bound to the cofactors FMN and AMP, the amino acids arginine and citrulline, the drug theophylline and the aminoglycoside antibiotic tobramycin in solution. Analysis of the structural features of these complexes allows the identification of molecular themes in RNA aptamer structure, recognition and discrimination.