Population Structure of the Fusarium graminearum Species Complex from a Single Japanese Wheat Field Sampled in Two Consecutive Years
Population Structure of the Fusarium graminearum Species Complex from a Single Japanese Wheat Field Sampled in Two Consecutive Years
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DOI：
10.1094/pdis-93-2-0170
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发表时间：
2009-02-01
期刊：
影响因子：
4.5
通讯作者：
Hyakumachi, M.
中科院分区：
文献类型：
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作者：
Karugia, G. W.;Suga, H.;Hyakumachi, M.
禾谷镰刀菌种复合体（Fusarium graminearum species complex，Fg complex）由至少11个遗传学上不同的种组成，是世界范围内小麦和大麦赤霉病（Fusarium head blight，FHB）的重要病原体。我们通过连续两年从日本熊本县500 m2的试验麦田中取样获得了Fg复合体的成员，并对它们进行了物种身份和蒽烯化学型的评估。单倍型多样性估计使用11个可变数目的串联重复序列（VNTR）标记。除了这两个样品（2003年的03 W组和2004年的04 W组）之外，本研究还分析了2002年在福冈县收获并种植在实验田中的种子（02 WSC组）和2003年在长崎收获并种植的种子（03 WSC组）的病原体种群。每组收集46株分离株。小麦穗上的大多数菌株被归类为亚洲镰孢（F asiaticum）;只有4个菌株被归类为狭义禾谷镰孢（F graminearum sensu stricto（s. str.）。在183株Fg复合菌中，NIV型80株（44%），而禾谷镰刀菌（Fg graminearum）4株均为NIV型103株（56%）。str.分离株均为3ADON型。本研究未检出15 ADON型分离株。03 W和04 W的单端孢霉烯化学型组成几乎相同。在所有群体中观察到东亚飞蝗的高基因多样性。根据低水平的固着指数（F-ST）和高水平的有效迁飞数（Nm），提出了不同的种群划分。在四个类群中未推断出亚洲种。这些结果表明，FHB在2003年和2004年的实验麦田是由一个遗传相似的人口，流行于熊本，福冈，和长崎县。
The Fusarium graminearum species complex (Fg complex) that consists of at least 11 phylogenetically distinct species contains important Fusarium head blight (FHB) pathogens of wheat and barley worldwide. We obtained members of the Fg complex by sampling from a 500-m(2) experimental wheat field in Kumamoto Prefecture, Japan in two consecutive years and assessed them for species identity and trichothecene chemotype. Haplotype diversity was estimated by using 11 variable numbers of tandem repeat (VNTR) markers. In addition to these two samples (group 03W in 2003 and group 04W in 2004), pathogen populations from seed that was harvested in Fukuoka Prefecture and planted in the experimental field in 2002 (group 02WSC) and pathogen populations from seed that was harvested in Nagasaki Prefecture and planted in 2003 (group 03WSC) were analyzed for this study. Forty-six isolates were collected in each group. Most isolates from wheat heads were classified as F asiaticum; only four isolates were classified as F graminearum sensu stricto (s. str.). Out of a total of 183 Fg complex strains, 80 isolates (44%) were of the NIV type, while 103 isolates (56%), including all four F graminearum s. str. isolates, were of the 3ADON type. No 15ADON type isolate was detected in this study. Trichothecene chemotype compositions of 03W and 04W were nearly identical. High gene diversity of E asiaticum was observed in all groups. Based on the observed low level of fixation index (F-ST) and high level of effective number of migrants (Nm), distinctive population subdivision of F. asiaticum was not inferred among the four groups. These results suggest that FHB in the experimental wheat field in both 2003 and 2004 was caused by a genetically similar population, which prevails in Kumamoto, Fukuoka, and Nagasaki prefectures.