Immuno-Analysis and FACS Sorting of Adult Muscle Fiber-Associated Stem/Precursor Cells
Immuno-Analysis and FACS Sorting of Adult Muscle Fiber-Associated Stem/Precursor Cells
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DOI：
10.1007/978-1-60761-063-2_11
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发表时间：
2010-01-01
期刊：
影响因子：
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通讯作者：
Conboy, Irina M.
中科院分区：
文献类型：
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作者：
Conboy, Michael J.;Cerletti, Massimiliano;Conboy, Irina M.
我们描述了我们通常用于检测更稳健的细胞标记物的免疫染色方法，这些细胞标记物可识别通过肌卫星细胞的酶促和/或机械解离获得的各种细胞群：CD 34、m-钙粘蛋白和Pax 7，表达CXCR 4和β 1-整联蛋白的自我更新肌肉干细胞，表达Pax 3、Pax 7、Myf 5、MyoD 1和结蛋白的增殖性肌源性祖细胞群，分化表达肌细胞生成素和cMHC的成肌细胞，以及浸润损伤和再生骨骼肌的表达CD 45的白细胞谱系细胞。
We describe the immunostaining methods we commonly use to detect the more robust cell markers identifying the various cell populations obtained by the enzymatic and or mechanical dissociation of muscle satellite cells: CD34, m-Cadherin, and Pax7, self-renewing muscle stem cells expressing CXCR4 and beta 1-integrin, populations of proliferative myogenic progenitor cells expressing, Pax3, Pax7, Myf5, MyoD1, and desmin, differentiating myoblasts expressing myogenin and cMHC, and the CD45 expressing leukocyte lineage cells that infiltrate injured and regenerating skeletal muscle.