Tissue expression of endothelin-1 mRNA in endotoxaemia
Tissue expression of endothelin-1 mRNA in endotoxaemia
复制标题
DOI：
10.1006/bbrc.1996.0115
复制
发表时间：
1996-01-26
影响因子：
3.1
通讯作者：
PooleWilson, PA
中科院分区：
文献类型：
--
作者：
Kaddoura, S;Curzen, NP;PooleWilson, PA
在脓毒症的动物模型中，循环内皮素-1浓度升高。内皮素-1的主要作用似乎是作为局部自分泌和旁分泌因子，而不是作为循环激素，血浆浓度可能不能反映局部组织的浓度。因此，我们通过核糖核酸酶保护试验测量了编码前内皮素-1的组织表达，作为体内内毒素血症大鼠局部产生ET-1的指标。同时观察地塞米松治疗前后的疗效。内毒素血症大鼠心、肺组织内毒素刺激后6h，内毒素血症组大鼠肺组织中前内皮素-1基因表达明显升高。肾脏和骨骼肌中的表达没有明显变化。地塞米松治疗后6h，心脏组织中前内皮素-1mRNA水平的升高明显减弱。因此，我们已经证明了内毒素对前内皮素-1mRNA表达的影响以及对地塞米松敏感性的组织特异性差异。这可能解释了局部血管对内毒素反应的一些组织间差异。(C)1996年学术出版社。
Circulating endothelin-1 concentrations are elevated in animal models of sepsis. The major actions of endothelin-1 appear to be as a local autocrine and paracrine factor, rather than as a circulating hormone, and plasma concentrations may not reflect local tissue concentrations. We therefore measured tissue expression of mRNA encoding pre-pro-endothelin-1 by RNase protection assays, as an indicator of local production of ET-1 in an in vivo rat model of endotoxaemia. The effects of dexamethasone pre-treatment were also examined. There was a tissue-specific increase in pre-pro-endothelin-1 mRNA in endotoxaemia, apparent at 6h after endotoxin challenge in heart and lung. No significant changes in expression were seen in kidney or skeletal muscle. Dexamethasone pre-treatment significantly attenuated the rise in pre-pro-endothelin-1 mRNA in heart at 6h. Therefore, we have demonstrated tissue-specific differences in the effect of endotoxin upon pre-pro-endothelin-1 mRNA expression and in sensitivity to dexamethasone. This could account for some of the inter-tissue differences seen in local vascular response to endotoxin. (C) 1996 Academic Press, Inc.