Towards a nondestructive chemical characterization of biofilm matrix by Raman microscopy
Towards a nondestructive chemical characterization of biofilm matrix by Raman microscopy
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DOI：
10.1007/s00216-008-2470-5
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发表时间：
2009-01-01
影响因子：
4.3
通讯作者：
Haisch, Christoph
中科院分区：
文献类型：
--
作者：
Ivleva, Natalia P.;Wagner, Michael;Haisch, Christoph
在这项研究中，评估了拉曼显微镜（RM）对生物膜基质（包括微生物成分和细胞外聚合物（EPS））进行无损化学分析的适用性。对各种参考样品（例如生物膜特异性多糖、蛋白质、微生物和封装细菌）的检查揭示了不同生物膜成分的特征频率区域和特定标记带。根据收到的数据，对多物种生物膜光谱中的拉曼谱带进行了分配。对不同多物种生物膜的研究表明，RM 可以将生物膜内的各种结构外观与其化学成分的变化相关联，并提供有关复杂生物膜基质的化学信息。生物膜的 RM 分析结果与共焦激光扫描显微镜 (CLSM) 获得的数据非常一致。因此，RM 是一种很有前途的工具，可用于对不同生物膜成分进行无标记化学表征。此外，将 RM 与 CLSM 分析相结合来研究不同环境条件下生长的生物膜可以为生物膜中复杂的结构/功能相关性提供新的见解。
In this study, the applicability of Raman microscopy (RM) for nondestructive chemical analysis of biofilm matrix, including microbial constituents and extracellular polymeric substances (EPS), has been assessed. The examination of a wide range of reference samples such as biofilm-specific polysaccharides, proteins, microorganisms, and encapsulated bacteria revealed characteristic frequency regions and specific marker bands for different biofilm constituents. Based on received data, the assignment of Raman bands in spectra of multispecies biofilms was performed. The study of different multispecies biofilms showed that RM can correlate various structural appearances within the biofilm to variations in their chemical composition and provide chemical information about a complex biofilm matrix. The results of RM analysis of biofilms are in good agreement with data obtained by confocal laser scanning microscopy (CLSM). Thus, RM is a promising tool for a label-free chemical characterization of different biofilm constituents. Moreover, the combination of RM with CLSM analysis for the study of biofilms grown under different environmental conditions can provide new insights into the complex structure/function correlations in biofilms.