Measurement of cartilage oligomeric matrix protein (COMP) in normal and diseased equine synovial fluids
Measurement of cartilage oligomeric matrix protein (COMP) in normal and diseased equine synovial fluids
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DOI：
10.1053/joca.2000.0367
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发表时间：
2001-02-01
影响因子：
7
通讯作者：
Carter, SD
中科院分区：
文献类型：
--
作者：
Misumi, K;Vilim, V;Carter, SD
目的：测定马滑膜液中软骨寡聚基质蛋白（COMP）的含量，并比较正常马和关节病马滑膜液中COMP的含量。研究了滑膜液中COMP降解与基质金属蛋白酶活性的关系。设计：以马关节软骨制备的COMP抗原和小鼠抗人COMP单克隆抗体（12C4）建立抑制酶联免疫吸附试验。定量分析了马正常和病变关节滑液中COMP的含量。用明胶降解酶联免疫吸附试验评价了金属蛋白酶在同一滑膜液中的活性。采用Western blotting对COMP片段进行定性评价。结果：马COMP浓度在62.5 ~ 2000 ng/ml范围内，ELISA法对COMP的抑制作用可靠。无菌性和败血性关节疾病患者关节液中的COMP值（分别为19.7+/-15.3和16.1+/-11.2马克/毫升）显著高于对照组(P
Objective: This study was designed to assay cartilage oligomeric matrix protein (COMP) in equine synovial fluids and to compare the concentration in synovial fluids from normal horses with joint diseased horses. The relationship between the COMP degradation and the matrix metalloproteinase activity in synovial fluids was also investigated.Design: Using COMP antigen prepared from equine articular cartilage and murine monoclonal antibody (12C4) raised against human COMP, an inhibition ELISA was developed. COMP in equine synovial fluids from normal and diseased joints was quantified. Metalloproteinase activities were evaluated in the same synovial fluids by a gelatin degradation ELISA. COMP fragments were evaluated qualitatively by Western blotting.Results: The COMP inhibition ELISA was reliable at concentrations of equine COMP between 62.5 and 2000 ng/ml. COMP values in joint fluids in both aseptic and septic joint disease (19.7+/-15.3 and 16.1+/-11.2 mug/ml, respectively) were significantly (P