The ratio of full length IgY to truncated IgY in immune complexes affects macrophage phagocytosis and the acute phase response of mallard ducks (Anas platyrhynchos)
The ratio of full length IgY to truncated IgY in immune complexes affects macrophage phagocytosis and the acute phase response of mallard ducks (Anas platyrhynchos)
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DOI：
10.1016/j.dci.2003.11.003
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发表时间：
2004-06-01
影响因子：
2.9
通讯作者：
Klasing, KC
中科院分区：
文献类型：
--
作者：
Humphrey, BD;Calvert, CC;Klasing, KC
鸭子产生全长IgY和截断的IgYDeltaFc亚型。IgY和IgY（DeltaFc）分别从大肠杆菌疫苗接种鸭中分离得到，用于制备大肠杆菌- ig免疫复合物（IC）。鸭单核细胞对IC的吞噬能力随IgYDeltaFc的比例直接降低（p < 0.001）。将含有IgY:IgY（DeltaFc）的IC分别以100:0、50:50、0:100和0:0的比例（仅大肠杆菌）静脉注射到幼稚的绿头鸭体内。注射后24 h， 0:100和100:0比例的肉鸭血浆血凝素水平均高于50:50和单独注射大肠杆菌的肉鸭（p < 0.05）。肝脏il -1 β mRNA水平也遵循类似的模式。脾脏il -1 β mRNA随IgY（DeltaFc）比例的升高而显著降低（p < 0.01）。因此，随着感染从急性进展到慢性，IgY（DeltaFc）可能会将对IC的反应从脾脏转移到肝脏。(C) 2003 Elsevier Ltd.版权所有。
Ducks produce a full length IgY and a truncated isoform (IgYDeltaFc). IgY and IgY(DeltaFc) were isolated from ducks vaccinated against Escherichia coli and used to make E.coli-Ig immune complexes (IC). Phagocytosis of IC by duck monocytes decreased directly with the proportion of IgYDeltaFc (p < 0.001). IC containing IgY:IgY(DeltaFc) at ratios of 100:0, 50:50, 0: 100, and 0:0 (E. coli alone) were injected intravenously into naive mallard ducks. At 24 h after injection, plasma hemopexin levels were higher in ducks given either the 0:100 ratio or the 100:0 ratio than those given 50:50 or E. coli alone (p < 0.05). Liver IL-1beta mRNA levels followed a similar pattern. Splenic IL-1beta mRNA decreased markedly as the proportion of IgY(DeltaFc) increased (p < 0.01). Thus, IgY(DeltaFc) may shift the response to IC from the spleen to the liver as infections progress from acute to chronic. (C) 2003 Elsevier Ltd. All rights reserved.