Distinct subfunctionalization and neofunctionalization of the B-class MADS-box genes in Physalis floridana
Distinct subfunctionalization and neofunctionalization of the B-class MADS-box genes in Physalis floridana
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DOI：
10.1007/s00425-014-2190-3
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发表时间：
2014-10
期刊：
影响因子：
4.3
通讯作者：
Shaohua Zhang;Jisi Zhang;J. Zhao;Chaoying He
中科院分区：
文献类型：
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作者：
Shaohua Zhang;Jisi Zhang;J. Zhao;Chaoying He
本研究的主要结论表明，在PhsalisPFGLO1-PFDEF中，PfGLLO1-PFDEF主要决定了花冠和雄蕊的特性，并在雌蕊群中获得了一个新的功能，而PFGLO2-PFTM6只在花粉成熟过程中起作用。两个GLOBOSA样(GLO样)的PFGLO1和PFGLO2和两个DEFICIENS样(DEF样)的PFDEF和PFTM6基因在多花酸浆中存在。然而，双层灯笼1(Dol1)突变是PFGLO1单一隐性突变的结果，暗示着B类基因的明显分化模式。在这项工作中，我们利用烟草Rattle病毒(TRV)介导的基因沉默方法进一步验证了这一假设。弗罗里达纳。PFGLO1或/和PFDEF的沉默显示了它们在决定花冠和雄蕊群特征方面的主要作用。然而，特异的PFGLO2或/和PFTM6沉默并不影响任何器官的特性，但显示出成熟花粉的减少。这些结果表明，PFGLO2和PFTM6都失去了在器官鉴定中的作用，但在花粉成熟中发挥了作用。对正反交结果的评估表明，PFGLO1和PFDEF可能在雌雄异株的功能中获得了一个重要的和新的作用。这种重复的GLO-DEF异源二聚体基因在花发育中的差异与茄科中现有的观察结果不同。因此，我们的研究为被子植物中复制的B类MADS-box基因的功能进化提供了新的线索。
Main conclusionThis work suggested that inPhysalisPFGLO1–PFDEFprimarily determined corolla and androecium identity, and acquired a novel role in gynoecia functionality, while PFGLO2–PFTM6 functioned in pollen maturation only.AbstractThe B-class MADS-box genes play a crucial role in determining the organ identity of the corolla and androecium. TwoGLOBOSA-like (GLO-like)PFGLO1andPFGLO2and twoDEFICIENS-like (DEF-like)PFDEFandPFTM6genes were present inPhysalis floridana. However, thedouble-layered-lantern1(doll1) mutant is the result of a single recessive mutation inPFGLO1, hinting a distinct divergent pattern of B-class genes. In this work, we utilized the tobacco rattle virus (TRV)-mediated gene silencing approach to further verify this assumption inP. floridana. Silencing ofPFGLO1or/andPFDEFdemonstrated their primary role in determining corolla and androecium identity. However, specificPFGLO2or/andPFTM6silencing did not affect any organ identity but showed a reduction in mature pollen. These results suggested that bothPFGLO2andPFTM6had lost their role in organ identity determination but functioned in pollen maturation. Evaluation of fruit setting in reciprocal crosses suggested that bothPFGLO1andPFDEFmight have acquired an essential and novel role in the functionality of gynoecia. Such a divergence of the duplicated GLO–DEF heterodimer genes in floral development is different from the existing observations within Solanaceae. Therefore, our research sheds new light on the functional evolution of the duplicated B-class MADS-box genes in angiosperms.