Ultradeep Pyrosequencing Detects Complex Patterns of CD8+ T-Lymphocyte Escape in Simian Immunodeficiency Virus-Infected Macaques
Ultradeep Pyrosequencing Detects Complex Patterns of CD8+ T-Lymphocyte Escape in Simian Immunodeficiency Virus-Infected Macaques
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DOI：
10.1128/jvi.00897-09
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发表时间：
2009-08-15
影响因子：
5.4
通讯作者：
O'Connor, David
中科院分区：
文献类型：
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作者：
Bimber, Benjamin N.;Burwitz, Benjamin J.;O'Connor, David
人类和猿类免疫缺陷病毒(HIV/SIV)在每个受感染的宿主中表现出巨大的序列异质性。在这里，我们使用超深焦测序来创建SIV感染的猕猴CD8(+)T淋巴细胞(CD8-TL)逃逸的全面图像，揭示了以前未发现的病毒变异的复杂模式。这种敏感度的提高使感染后17天就能检测到急性CD8-TL逃逸，这是直肠内感染的猕猴中最早公布的CD8-TL逃逸的例子。这些数据表明焦磷酸测序可以用来研究免疫缺陷病毒感染过程中CD8-TL逃逸的进化，具有前所未有的敏感性。
Human and simian immunodeficiency viruses (HIV/SIV) exhibit enormous sequence heterogeneity within each infected host. Here, we use ultradeep pyrosequencing to create a comprehensive picture of CD8(+) T-lymphocyte (CD8-TL) escape in SIV-infected macaques, revealing a previously undetected complex pattern of viral variants. This increased sensitivity enabled the detection of acute CD8-TL escape as early as 17 days postinfection, representing the earliest published example of CD8-TL escape in intrarectally infected macaques. These data demonstrate that pyrosequencing can be used to study the evolution of CD8-TL escape during immunodeficiency virus infection with an unprecedented degree of sensitivity.