Characterization of the Complete Mitochondrial Genome of Cerura menciana and Comparison with Other Lepidopteran Insects.
Characterization of the Complete Mitochondrial Genome of Cerura menciana and Comparison with Other Lepidopteran Insects.
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DOI：
10.1371/journal.pone.0132951
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Liu C
中科院分区：
文献类型：
--
作者：
Dai L;Qian C;Zhang C;Wang L;Wei G;Li J;Zhu B;Liu C
本研究对 Cerura menciana（鳞翅目：Notodontidae）的完整线粒体基因组（线粒体基因组）进行了测序和分析。线粒体基因组是一个 15,369 bp 的环状分子，包含 13 个蛋白质编码基因 (PCG)、2 个核糖体 RNA (rRNA) 基因、22 个转移 RNA (tRNA) 基因和一个富含 A+T 的区域。正 AT 偏度 (0.031) 表明存在的 As 多于 T。除细胞色素 c 氧化酶亚基 1 (cox1) 基因由 CAG 启动外，所有 PCG 均由 ATN 密码子启动。 13 个 PCG 中的两个含有不完整终止密码子 T 或 TA，而其他则以终止密码子 TAA 终止。富含 A+T 的区域长度为 372 bp，由“ATAGA”基序组成，后跟 18 bp 的多聚 T 片段、微卫星样 (AT)8 和 trnM 基因上游的多聚 A 元件。检查密码子使用情况的结果表明，Asn、Ile、Leu2、Lys、Tyr 和 Phe 是六种最常见的氨基酸，而 Cys 是最罕见的。根据其他昆虫有丝分裂基因组的 13 个 PCG 的核苷酸序列进行系统发育关系分析，证实 C. menciana 属于 Notodontidae 科。
The complete mitochondrial genome (mitogenome) of Cerura menciana (Lepidoptera: Notodontidae) was sequenced and analyzed in this study. The mitogenome is a circular molecule of 15,369 bp, containing 13 protein-coding genes (PCGs), two ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes and a A+T-rich region. The positive AT skew (0.031) indicated that more As than Ts were present. All PCGs were initiated by ATN codons, except for the cytochrome c oxidase subunit 1 (cox1) gene, which was initiated by CAG. Two of the 13 PCGs contained the incomplete termination codon T or TA, while the others were terminated with the stop codon TAA. The A+T-rich region was 372 bp in length and consisted of an ‘ATAGA’ motif followed by an 18 bp poly-T stretch, a microsatellite-like (AT)8 and a poly-A element upstream of the trnM gene. Results examining codon usage indicated that Asn, Ile, Leu2, Lys, Tyr and Phe were the six most frequently occurring amino acids, while Cys was the rarest. Phylogenetic relationships, analyzed based on the nucleotide sequences of the 13 PCGs from other insect mitogenomes, confirmed that C. menciana belongs to the Notodontidae family.