Mapping cancer cell metabolism with(13)C flux analysis: Recent progress and future challenges.
Mapping cancer cell metabolism with(13)C flux analysis: Recent progress and future challenges.
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DOI：
10.4103/1477-3163.115422
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发表时间：
2013
影响因子：
--
通讯作者：
Young JD
中科院分区：
文献类型：
--
作者：
Duckwall CS;Murphy TA;Young JD
能量代谢的重编程正在成为癌细胞的重要分子标志。最近的发现将特定的代谢改变与癌症发展联系起来，加强了这样一种观点，即代谢改变不仅仅是恶性转化的副作用，实际上可能是某些癌症中肿瘤生长和进展的功能驱动因素。因此，失调的代谢途径已成为癌症治疗的有吸引力的靶标。本文综述了13 C代谢通量分析（MFA）在肿瘤细胞内碳流代谢中的应用。我们总结了MFA的几个最近的应用程序，已经确定了新的生物合成途径参与癌细胞增殖，并阐明了特定的癌基因在调节这些途径的作用。通过这些研究，很明显，癌细胞的代谢表型并不像以前认为的那样同质，而是强烈依赖于每种情况下起作用的分子改变和环境因素。
The reprogramming of energy metabolism is emerging as an important molecular hallmark of cancer cells. Recent discoveries linking specific metabolic alterations to cancer development have strengthened the idea that altered metabolism is more than a side effect of malignant transformation, but may in fact be a functional driver of tumor growth and progression in some cancers. As a result, dysregulated metabolic pathways have become attractive targets for cancer therapeutics. This review highlights the application of13C metabolic flux analysis (MFA) to map the flow of carbon through intracellular biochemical pathways of cancer cells. We summarize several recent applications of MFA that have identified novel biosynthetic pathways involved in cancer cell proliferation and shed light on the role of specific oncogenes in regulating these pathways. Through such studies, it has become apparent that the metabolic phenotypes of cancer cells are not as homogeneous as once thought, but instead depend strongly on the molecular alterations and environmental factors at play in each case.