ASAP, a human microtubule-associated protein required for bipolar spindle assembly and cytokinesis
ASAP, a human microtubule-associated protein required for bipolar spindle assembly and cytokinesis
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DOI：
10.1073/pnas.0500964102
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发表时间：
2005-08-09
影响因子：
11.1
通讯作者：
Rouquier, S
中科院分区：
文献类型：
--
作者：
Saffin, JM;Venoux, M;Rouquier, S
我们已经鉴定出一种独特的人类微管相关蛋白（MAP），命名为ASAP （ASter-Associated protein）。ASAP在间期定位于微管，在有丝分裂时与有丝分裂纺锤体结合，在细胞分裂时定位于中央体，并通过其cooh末端结构域直接与纯化的微管结合。在间期细胞中，ASAP的过度表达引起细胞质微管的深度捆绑，在有丝分裂中引起异常的单极纺锤体。RNA干扰导致ASAP耗竭导致严重的有丝分裂缺陷：它引起有丝分裂纺锤体异常，延迟有丝分裂进程，导致细胞分裂缺陷或细胞死亡。这些结果表明ASAP在双极性有丝分裂纺锤体的组织、有丝分裂进程和细胞分裂中起着至关重要的作用，并将ASAP定义为纺锤体正确组装的关键因素。
We have identified a unique human microtubule-associated protein (MAP) named ASAP for ASter-Associated Protein. ASAP localizes to microtubules in interphase, associates with the mitotic spindle during mitosis, localizes to the central body during cytokinesis and directly binds to purified microtubules by its COOH-terminal domain. Overexpression of ASAP induces profound bundling of cytoplasmic microtubules in interphase cells and aberrant monopolar spindles in mitosis. Depletion of ASAP by RNA interference results in severe mitotic defects: it provokes aberrant mitotic spindle, delays mitotic progression, and leads to defective cytokinesis or cell death. These results suggest a crucial role for ASAP in the organization of the bipolar mitotic spindle, mitosis progression, and cytokinesis and define ASAP as a key factor for proper spindle assembly.