Understanding of Waggle Dance in the Honey Bee (Apis mellifera) from the Perspective of Long Non-Coding RNA.
Understanding of Waggle Dance in the Honey Bee (Apis mellifera) from the Perspective of Long Non-Coding RNA.
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DOI：
10.3390/insects13020111
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发表时间：
2022-01-19
期刊：
影响因子：
3
通讯作者：
Su S
中科院分区：
文献类型：
--
作者：
Feng W;Huang J;Zhang Z;Nie H;Lin Y;Li Z;Su S
蜜蜂（Apis mellifera）的舞蹈行为非常复杂且独特的行为模式，确保了食物信息的有效和高质量的交流。蜜蜂的舞蹈行为已经被发现并阐述了几十年，但这种行为背后的调控机制仍不清楚。在这项研究中，通过改变食物质量，例如蔗糖溶液的浓度，我们成功地操纵了蜜蜂的舞蹈行为。然后我们研究了蜜蜂大脑中的长非编码 RNA (lncRNA) 及其靶基因对摇摆舞的影响。结果表明，摇摆舞者和非跳舞蜜蜂大脑中的lncRNA表现出显着差异。此外，lncRNA-mRNA关联分析表明大脑中的信号转导可能参与了摇摆舞的调节。我们的研究结果表明，神经递质可能在摇摆舞中充当了信使的角色。这是首次从长链非编码RNA的角度研究蜜蜂的摇摆舞。总而言之，这项研究有望为探索行为与大脑之间的关系提供一条新途径。自从卡尔·冯·弗里施发现并解释了蜜蜂的“舞蹈语言”以来，舞蹈行为的行为学研究已经取得了一些发现。然而，长链非编码RNA对舞蹈行为的功能和作用至今仍知之甚少。在本研究中，通过RNA测序分析了摇摆舞者和非跳舞蜜蜂大脑中lncRNA的差异表达模式。此外，还构建了 lncRNA-mRNA 关联分析来破译摇摆舞。 RNA测序结果表明，从蜜蜂大脑中总共检测到2877个lncRNA和9647个mRNA。进一步的比较分析显示，两条lncRNA MSTRG.6803.3和XR_003305156.1可能参与了摇摆舞。 lncRNA-mRNA关联分析表明，摇摆舞者和非跳舞蜜蜂大脑中差异表达lncRNA的靶基因主要注释在与代谢过程、信号传导和刺激反应相关的生物过程以及与信号转导活性、分子转导活性和结合相关的分子功能中。氮代谢可能与摇摆舞的调节有关。我们的发现有助于进一步了解摇摆舞的发生和发展。
Dance behaviour of honey bee (Apis mellifera) is highly sophisticated and a unique behavioural pattern that ensures effective and high-quality communication of food information. Honey bee dance behaviour has been discovered and elaborated for decades, but the regulatory mechanism underlying this behaviour is still unclear. In this study, by varying the food quality, such as the concentration of sucrose solution, we successfully manipulated the dance behaviour of honey bees. Then we investigated the effect of long non-coding RNAs (lncRNAs) and their target genes in honey bee brains on waggle dance. The results indicated that lncRNAs in brains of waggle dancers and non-dancing bees exhibited significant differences. Furthermore, lncRNA-mRNA association analysis showed that signal transduction in the brain may be participated in the modulation of waggle dance. Our findings suggested that neurotransmitters presumably served as messengers in the waggle dance. It is the first time that the waggle dance in the honey bee is studied from the perspective of long non-coding RNA. Taken together, this study is expected to provide a new pathway to explore the relationship between behaviour and brain. The ethological study of dance behaviour has yielded some findings since Karl Von Frisch discovered and interpreted the ‘dance language’ in the honey bee. However, the function and role of long non-coding RNAs on dance behaviour are hardly known until now. In this study, the differential expression patterns of lncRNAs in the brains of waggling dancers and non-dancing bees were analysed by RNA sequencing. Furthermore, lncRNA-mRNA association analysis was constructed to decipher the waggle dance. The results of RNA sequencing indicated that a total of 2877 lncRNAs and 9647 mRNAs were detected from honey bee brains. Further comparison analysis displayed that two lncRNAs, MSTRG.6803.3 and XR_003305156.1, may be involved in the waggle dance. The lncRNA-mRNA association analysis showed that target genes of differentially expressed lncRNAs in the brains between waggling dancers and non-dancing bees were mainly annotated in biological processes related to metabolic process, signalling and response to stimulus and in molecular function associated with signal transducer activity, molecular transducer activity and binding. Nitrogen metabolism was likely implicated in the modulation of the waggle dance. Our findings contribute to further understanding the occurrence and development of waggle dance.
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影响因子：
4.5
作者：
Kapusta A;Kronenberg Z;Lynch VJ;Zhuo X;Ramsay L;Bourque G;Yandell M;Feschotte C
通讯作者：
Feschotte C
DOI：
10.1073/pnas.0610506104
发表时间：
2007-01-30
影响因子：
11.1
作者：
Barron, Andrew B.;Maleszka, Ryszard;Robinson, Gene E.
通讯作者：
Robinson, Gene E.
DOI：
10.1016/s1095-6433(98)10094-6
发表时间：
1998-08-01
影响因子：
2.3
作者：
Bozic, J;Woodring, J
通讯作者：
Woodring, J
影响因子：
2.2
作者：
FOX, PM;LARSEN, JR
通讯作者：
LARSEN, JR
影响因子：
3.7
作者：
Alaux C;Duong N;Schneider SS;Southey BR;Rodriguez-Zas S;Robinson GE
通讯作者：
Robinson GE