The overexpression of a maize mitogen-activated protein kinase gene (ZmMPK5) confers salt stress tolerance and induces defence responses in tobacco.
The overexpression of a maize mitogen-activated protein kinase gene (ZmMPK5) confers salt stress tolerance and induces defence responses in tobacco.
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DOI：
10.1111/plb.12084
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发表时间：
2014-05
期刊：
影响因子：
3.9
通讯作者：
Dongyun Zhang;Shanshan Jiang;J. Pan;X. Kong;Yan Zhou;Yukun Liu;Dapeng Li
中科院分区：
文献类型：
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作者：
Dongyun Zhang;Shanshan Jiang;J. Pan;X. Kong;Yan Zhou;Yukun Liu;Dapeng Li
作为固着生物，植物面临着潜在的危险，包括多种生物和非生物胁迫。丝裂原活化蛋白激酶（MAPK）是参与这些过程的一种通用信号通路。先前的研究表明，玉米ZmMPK 5在转录和翻译后水平上被各种刺激诱导。本研究将ZmMPK 5基因在烟草中进行过表达，以进一步分析其生物学功能。在盐和氧化胁迫下，ZmMPK 5过表达株系表现出较轻的损伤和较强的生长表型，对应于一系列生理变化。此外，转基因株系积累较少的活性氧（ROS），并有较高水平的抗氧化酶活性和代谢产物比野生型（WT）植物NaCl处理后。定量RT-PCR结果表明，转基因植株中ROS相关基因和逆境响应基因的表达量高于野生型植株。此外，转基因株系表现出对病毒病原体的抗性增强，并组成性地表达更高的病程相关基因的转录水平，如PR 1a，PR 4，PR 5和EREBP。综上所述，这些结果表明，ZmMPK 5参与盐胁迫，氧化胁迫和病原体防御信号通路，其功能可能至少部分致力于有效地消除盐胁迫下ROS的积累。
As sessile organisms, plants are exposed to potential dangers, including multiple biotic and abiotic stresses. The mitogen-activated protein kinase (MAPK) is a universal signalling pathways involved in these processes. A previous study showed that maize ZmMPK5 is induced by various stimuli at transcriptional and post-translational levels. In this study, ZmMPK5 was overexpressed in tobacco to further analyse its biological functions. Under salt and oxidative stresses, ZmMPK5-overexpressing lines displayed less severe damage and stronger growth phenotypes corresponding to a series of physiological changes. In addition, the transgenic lines accumulated less reactive oxygen species (ROS) and had higher levels of antioxidant enzyme activity and metabolites than wild-type (WT) plants following NaCl treatment. Quantitative RT-PCR revealed that the expression of ROS-related and stress-responsive genes was higher in transgenic plants than in WT plants. Furthermore, transgenic lines exhibited enhanced resistance to viral pathogens, and expressed constitutively higher transcript levels of pathogenesis-related genes, such as PR1a, PR4, PR5 and EREBP. Taken together, these results demonstrated that ZmMPK5 is involved in salt stress, oxidative stress and pathogen defence signalling pathways, and its function may be at least partly devoted to efficiently eliminating ROS accumulation under salt stress.