Differential cellular expression of galectin family mRNAs in the epithelial cells of the mouse digestive tract
Differential cellular expression of galectin family mRNAs in the epithelial cells of the mouse digestive tract
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DOI：
10.1369/jhc.5a6685.2005
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发表时间：
2005-11-01
影响因子：
3.2
通讯作者：
Iwanaga, T
中科院分区：
文献类型：
--
作者：
Nio, J;Kon, Y;Iwanaga, T
半乳糖凝集素是一种动物凝集素，能识别糖结合物的β-半乳糖苷，在肠道中含量丰富。本研究通过原位杂交揭示了半乳糖凝集素亚型在小鼠消化道的细胞表达。5个亚型(Galectin-2、-3、-4/6和-7)的信号仅在上皮细胞中检测到。在腺胃中，Galectin-2和Galectin-4/6主要表达于胃小凹至胃腺颈，其中粘液细胞是主要的细胞来源。小肠表现出与成熟相关的Galectin-2、-3和-4/6mRNAs的强烈表达。Galectin-2从隐窝到绒毛底部有较强的表达，而Galectin-3的转录集中在绒毛顶端。Galectin-4/6在绒毛下半部表达最强。Galectin-2在小肠的杯状细胞中也有表达，而在大肠的杯状细胞中不表达。在大肠中，Galectin-4/6占优势，隐窝的上半部分同时含有Galectin-3的转录本。从唇部到前胃和肛门的复层上皮强表达Galectin-7，弱表达Galectin-3。由于消化道中的Galectins可能是多功能的，了解其细胞/阶段特异性表达的信息有助于更好地了解Galectins的功能和病理参与。
Galectin is an animal lectin that recognizes beta-galactosides of glycoconjugates and is abundant in the gut. This study revealed the cellular expression of galectin subtypes throughout the mouse digestive tract by in situ hybridization. Signals for five subtypes (galectin-2, -3, -4/6, and -7) were detected exclusively in the epithelia. In the glandular stomach, galectin-2 and -4/6 were predominantly expressed from gastric pits to neck of gastric glands, where mucous cells were the main cellular sources. The small intestine exhibited intense, maturation-associated expressions of galectin-2, -3, and -4/6 mRNAs. Galectin-2 was intensely expressed from crypts to the base of villi, whereas transcripts of galectin-3 gathered at villous tips. Signals for galectin-4/6 were most intense at the lower half of villi. Galectin-2 was also expressed in goblet cells of the small intestine but not in those of the large intestine. In the large intestine, galectin-4/6 predominated, and the upper half of crypts simultaneously contained transcripts of galectin-3. Stratified epithelium from the lip to forestomach and anus intensely expressed galectin-7 with weak expressions of galectin-3. Because galectins in the digestive tract may be multi-functional, information on their cell/ stage-specific expression contributes to a better understanding of the functions and pathological involvements of galectins.