Detection of glioma infiltration at the tumor margin using quantitative stimulated Raman scattering histology.
Detection of glioma infiltration at the tumor margin using quantitative stimulated Raman scattering histology.
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DOI：
10.1038/s41598-021-91648-8
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发表时间：
2021-06-09
影响因子：
4.6
通讯作者：
Hervey-Jumper SL
中科院分区：
文献类型：
--
作者：
Pekmezci M;Morshed RA;Chunduru P;Pandian B;Young J;Villanueva-Meyer JE;Tihan T;Sloan EA;Aghi MK;Molinaro AM;Berger MS;Hervey-Jumper SL
在弥漫性胶质瘤的治疗中，浸润边缘肿瘤的识别和切除仍然是一个核心挑战。先前的工作已经证明，荧光标记工具和射线照相成像是有用的外科手术佐剂与宏观分辨率。然而，它们在肿瘤边缘失去灵敏度，并且对于较低级别的组织学具有有限的临床效用。基于光纤激光的受激拉曼组织学（SRH）是一种光学成像技术，其提供未处理组织的显微组织表征。从浸润性胶质瘤边缘提取的组织的SRH是否能识别显微镜下的残留病变仍是未知数。在这里，我们获得了胶质瘤边缘标本SRH，组织学和肿瘤特异性组织表征。广义线性混合模型用于评价一致性。我们发现，SRH在167个切缘标本中的82个（49%）中确定了残留肿瘤，相比之下，IHC在128个样本中的72个（56%）中确定了残留肿瘤，H&E在169个样本中的82个（49%）中确定了残留肿瘤。所有3种模式之间的观察者内一致性得到证实。这些数据表明，SRH检测残留的显微肿瘤浸润性胶质瘤边缘，并可能是一个有前途的工具，以提高切除范围。
In the management of diffuse gliomas, the identification and removal of tumor at the infiltrative margin remains a central challenge. Prior work has demonstrated that fluorescence labeling tools and radiographic imaging are useful surgical adjuvants with macroscopic resolution. However, they lose sensitivity at the tumor margin and have limited clinical utility for lower grade histologies. Fiber-laser based stimulated Raman histology (SRH) is an optical imaging technique that provides microscopic tissue characterization of unprocessed tissues. It remains unknown whether SRH of tissues taken from the infiltrative glioma margin will identify microscopic residual disease. Here we acquired glioma margin specimens for SRH, histology, and tumor specific tissue characterization. Generalized linear mixed models were used to evaluate agreement. We find that SRH identified residual tumor in 82 of 167 margin specimens (49%), compared to IHC confirming residual tumor in 72 of 128 samples (56%), and H&E confirming residual tumor in 82 of 169 samples (49%). Intraobserver agreements between all 3 modalities were confirmed. These data demonstrate that SRH detects residual microscopic tumor at the infiltrative glioma margin and may be a promising tool to enhance extent of resection.
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影响因子：
4.1
作者：
Lau, Darryl;Hervey-Jumper, Shawn L.;Berger, Mitchel S.
通讯作者：
Berger, Mitchel S.
影响因子：
28.4
作者：
Brown TJ;Brennan MC;Li M;Church EW;Brandmeir NJ;Rakszawski KL;Patel AS;Rizk EB;Suki D;Sawaya R;Glantz M
通讯作者：
Glantz M
影响因子：
45.3
作者：
Smith, Justin S.;Chang, Edward F.;Berger, Mitchel S.
通讯作者：
Berger, Mitchel S.
影响因子：
4.1
作者：
Orringer, Daniel;Lau, Darryl;Sagher, Oren
通讯作者：
Sagher, Oren
DOI：
10.1097/nen.0b013e31820565f9
发表时间：
2011-02
影响因子：
3.2
作者：
Camelo-Piragua S;Jansen M;Ganguly A;Kim JC;Cosper AK;Dias-Santagata D;Nutt CL;Iafrate AJ;Louis DN
通讯作者：
Louis DN