Expression of Lex antigen in Schistosoma japonicum and S-haematobium and immune responses to Lex in infected animals:: lack of Lex expression in other trematodes and nematodes
Expression of Lex antigen in Schistosoma japonicum and S-haematobium and immune responses to Lex in infected animals:: lack of Lex expression in other trematodes and nematodes
复制标题
DOI：
10.1093/glycob/8.6.615
复制
发表时间：
1998-06-01
期刊：
影响因子：
4.3
通讯作者：
Cummings, RD
中科院分区：
文献类型：
--
作者：
Nyame, AK;Debose-Boyd, R;Cummings, RD
人类寄生吸虫曼氏血吸虫（Schistosoma mansoni）的成虫在人类中引起肝脾/肠并发症，其合成含有刘易斯x（Le（x））Gal β 1->4（Fuc α 1->3）GlcNAc β 1->R但不含唾液酸化刘易斯x（sLe（x））抗原的糖缀合物。我们现在报告我们的分析Le（x）和sLe（x）在S.haematobium和S.japonicum中的表达，这是另外两种引起疾病的人类染色体的主要物种，以及感染个体对这些抗原可能的自身免疫。通过ELISA和Western印迹分析使用单克隆抗体的寄生虫的洗涤剂提取物来评估抗原表达。血吸虫和日本血吸虫的几种高分子量糖蛋白均含有Le（x）抗原，但未检测到唾液酸化Le（x）抗原。此外，感染埃及血吸虫和日本血吸虫的人类和啮齿动物的血清含有与Le（x）反应的抗体。这些结果使我们研究Le（x）抗原是否在其他蠕虫中表达，包括寄生吸虫肝片吸虫、寄生线虫犬心丝虫、反刍线虫捻转血矛线虫和自由生活的线虫秀丽隐杆线虫。在这些其他蠕虫中，Le（x）和sialyl-Le（x）都检测不到。此外，蠕虫，包括寄生虫，都不表达Le（a）、Le（B）、Le（y）或H-1型抗原。然而，来自分析的所有蠕虫的几种糖蛋白被Lotus tetragonolobus凝集素和紫藤花凝集素结合，Lotus tetragonolobus凝集素结合Fuc α 1->3GlcNAc，紫藤花凝集素结合GalNAc β 1->4GlcNAc（lacdiNAc或LDN）。因此，血吸虫在蠕虫中表达Le（x）抗原方面可能是独特的，而许多不同的蠕虫可能表达α 1，3-岩藻糖基化聚糖和LDN基序。
Adults of the human parasitic trematode Schistosoma mansoni, which causes hepatosplenic/intestinal complications in humans, synthesize glycoconjugates containing the Lewis x (Le(x)) Gal beta 1-->4(Fuc alpha 1-->3)GlcNAc beta 1-->R, but not sialyl Lewis x (sLe(x)), antigen. We now report on our analyses of Le(x) and sLe(x) expression in S.haematobium and S.japonicum, which are two other major species of human schistosomes that cause disease, and the possible autoimmunity to these antigens in infected individuals. Antigen expression was evaluated by both ELISA and Western blot analyses of detergent extracts of parasites using monoclonal antibodies. Several high molecular weight glycoproteins in both S.haematobium and S.japonicum contain the Le(x) antigen, but no sialyl Le(x) antigen was detected. In addition, sera from humans and rodents infected with S.haematobium and S.japonicum contain antibodies reactive with Le(x). These results led us to investigate whether Le(x) antigens are expressed in other helminths, including the parasitic trematode Fasciola hepatica, the parasitic nematode Dirofilaria immitis (dog heartworm), the ruminant nematode Haemonchus contortus, and the free-living nematode Caenorhabditis elegans. Neither Le(x) nor sialyl-Le(x) is detectable in these other helminths. Furthermore, none of the helminths, including schistosomes, express Le(a), Le(b), Le(y) or the H-type 1 antigen. However, several glycoproteins from all helminths analyzed are bound by Lotus tetragonolobus agglutinin, which binds Fuc alpha 1-->3GlcNAc, and Wisteria floribunda agglutinin, which binds GalNAc beta 1-->4GlcNAc (lacdiNAc or LDN). Thus, schistosomes may be unique among helminths in expressing the Le(x) antigen, whereas many different helminths may express alpha 1,3-fucosylated glycans and the LDN motif.