Immunization with a 22-kDa outer membrane protein elicits protective immunity to multidrug-resistant Acinetobacter baumannii.
Immunization with a 22-kDa outer membrane protein elicits protective immunity to multidrug-resistant Acinetobacter baumannii.
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使用 22 kDa 外膜蛋白进行免疫可引发针对多重耐药鲍曼不动杆菌的保护性免疫。
DOI：
10.1038/srep20724
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发表时间：
2016-02-08
影响因子：
4.6
通讯作者：
Ma Y
中科院分区：
文献类型：
--
作者：
Huang W;Yao Y;Wang S;Xia Y;Yang X;Long Q;Sun W;Liu C;Li Y;Chu X;Bai H;Yao Y;Ma Y
A.随着多药耐药菌株和极端耐药菌株的迅速出现，鲍曼不动杆菌感染正成为越来越严重的健康问题，因此，迫切需要开发基于非抗生素的干预策略。本研究旨在鉴定分子量约为22 kDa的外膜蛋白Omp22是否具有成为有效疫苗候选物和对抗A.鲍曼不动杆菌感染Omp22分子长度为217个氨基酸，在已报道的851个A.鲍曼不动杆菌。重组Omp22在小鼠中有效地引发高滴度的特异性IgG。Omp 22的主动和被动免疫均可提高小鼠的存活率，抑制器官和外周血中的细菌负荷，并降低血清炎症细胞因子和趋化因子的水平。体外调理吞噬实验表明，Omp 22抗血清对不同克隆的临床A.鲍曼不动杆菌分离株，这是部分补体依赖性和调理吞噬作用。此外，高达500 μg的Omp 22给药未引起小鼠明显的病理变化。结论：Omp22是一种新的保守抗原，可用于研制有效的疫苗或抗血清。鲍曼不动杆菌感染。
A. baumannii infections are becoming more and more serious health issues with rapid emerging of multidrug and extremely drug resistant strains, and therefore, there is an urgent need for the development of nonantibiotic-based intervention strategies. This study aimed at identifying whether an outer membrane protein with molecular weight of about 22 kDa (Omp22) holds the potentials to be an efficient vaccine candidate and combat A. baumannii infection. Omp22 which has a molecule length of 217 amino acids kept more than 95% conservation in totally 851 reported A. baumannii strains. Recombinant Omp22 efficiently elicited high titers of specific IgG in mice. Both active and passive immunizations of Omp22 increased the survival rates of mice, suppressed the bacterial burdens in the organs and peripheral blood, and reduced the levels of serum inflammatory cytokines and chemokines. Opsonophagocytosis assays showed in vitro that Omp22 antiserum had highly efficient bactericidal activities on clonally distinct clinical A. baumannii isolates, which were partly complements-dependent and opsonophagocytic killing effects. Additionally, administration with as high as 500 μg of Omp22 didn’t cause obvious pathological changes in mice. In conclusion, Omp22 is a novel conserved and probably safe antigen for developing effective vaccines or antisera to control A. baumannii infections.