Chitinase producing bacteria with direct algicidal activity on marine diatoms.
Chitinase producing bacteria with direct algicidal activity on marine diatoms.
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对海洋硅藻具有直接杀藻活性的产生几丁质酶的细菌
DOI：
10.1038/srep21984
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发表时间：
2016-02-23
影响因子：
4.6
通讯作者：
Zheng T
中科院分区：
文献类型：
--
作者：
Li Y;Lei X;Zhu H;Zhang H;Guan C;Chen Z;Zheng W;Fu L;Zheng T
几丁质酶产生菌通过降解和利用几丁质，广泛参与水环境中的营养循环和能量流动。众所周知，硅藻细胞被由几丁质组成的盒状硅藻壳包裹。因此，硅藻中的几丁质是产几丁质酶细菌的天然靶标，然而，迄今为止，这两种有机基团之间的相互作用尚未研究。因此，本研究对一株几丁质酶产生菌（菌株LY03）对海链藻的溶藻机理进行了研究。首先研究了菌株LY 03的溶藻范围和溶藻方式，进而确定了菌株的生活力、趋化能力以及细菌与硅藻的直接相互作用特性。最后，对胞内溶藻物质的特性进行了鉴定，并确定了溶藻细菌细胞对藻细胞具有趋化性，利用鞭毛将自身固定在藻细胞上，产生几丁质酶降解藻细胞壁，最终导致藻细胞溶解和死亡的溶藻机理。首次研究了几丁质酶产生菌与硅藻之间的相互作用，证实了这种新的特殊的相互作用模式，为硅藻资源的保护和利用提供了理论依据。
Chitinase producing bacteria can involve extensively in nutrient cycling and energy flow in the aquatic environment through degradation and utilization of chitin. It is well known that diatoms cells are encased by box-like frustules composed of chitin. Thus the chitin containing of diatoms shall be a natural target of chitinase producing bacteria, however, the interaction between these two organismic groups has not been studied thus far. Therefore, in this study, the algicidal mechanism of one chitinase producing bacterium (strain LY03) onThalassiosira pseudonanawas investigated. The algicidal range and algicidal mode of strain LY03 were first studied and then bacterial viability, chemotactic ability and direct interaction characteristic between bacteria and diatom were also confirmed. Finally, the characteristic of the intracellular algicidal substance was identified and the algicidal mechanism was determined whereby algicidal bacterial cells showed chemotaxis to algal cells, fastened themselves on algal cells with their flagella and then produced chitinase to degrade algal cell walls and eventually caused algal lysis and death. It is the first time to investigate the interaction between chitinase producing bacteria and diatoms and this novel special interaction mode was confirmed in this study, which will be helpful in protection and utilization of diatoms resources.