Suppression of fructokinase encoded by LeFRK2 in tomato stem inhibits growth and causes wilting of young leaves
Suppression of fructokinase encoded by LeFRK2 in tomato stem inhibits growth and causes wilting of young leaves
复制标题
DOI：
10.1046/j.1365-313x.2003.01765.x
复制
发表时间：
2003-06-01
期刊：
影响因子：
7.2
通讯作者：
Granot, D
中科院分区：
文献类型：
--
作者：
German, MA;Dai, N;Granot, D
果糖激酶催化植物果糖磷酸化的关键步骤。LeFRK2是番茄植物中主要的果糖激酶编码基因，在根、茎和果实中大量表达。为了分析LeFRK2在植物发育中的作用，我们分析了具有StFRK（LeFRK2的马铃薯同源物）的正义和反义表达的转基因番茄植物。增加果糖激酶活性没有影响。然而，植物中的LeFRK2特异性抑制，无论是通过反义抑制或通过共抑制，表现出生长抑制和萎蔫的幼叶在白天。嫁接实验表明，反义植物的茎中间砧足以抑制生长并导致叶片萎蔫。茎次生木质部表现出特定的抑制LeFRK2和活性木质部的面积，估计曙红吸收，是显着较小的反义茎相比，野生型植物。这些结果表明，LeFRK2可能是影响生长和萎蔫的木质部的适当发育所必需的。
Fructokinases catalyze the key step of fructose phosphorylation in plants. LeFRK2 , the major fructokinase-encoding gene in tomato plants, is abundantly expressed in roots, stems, and fruits. To analyze the role of LeFRK2 in plant development, we analyzed transgenic tomato plants with sense and antisense expression of StFRK , the potato homolog of LeFRK2. Increased fructokinase activity had no effect. However, plants in which LeFRK2 was specifically suppressed, either via antisense suppression or via co-suppression, exhibited growth inhibition and wilting of young leaves at daytime. Grafting experiments indicated that a stem interstock of antisense plants was sufficient to inhibit growth and cause leaf wilting. Stem secondary xylem exhibited particular suppression of LeFRK2 and the area of active xylem, estimated by eosin uptake, was significantly smaller in antisense stem compared to that of wild-type plants. These results suggest that LeFRK2 might be required for proper development of xylem that affected growth and wilting.