USE OF NONDEFECTIVE ADENOVIRUS SIMIAN VIRUS 40 HYBRIDS FOR MAPPING SIMIAN VIRUS 40 GENOME
USE OF NONDEFECTIVE ADENOVIRUS SIMIAN VIRUS 40 HYBRIDS FOR MAPPING SIMIAN VIRUS 40 GENOME
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DOI：
10.1128/jvi.12.3.643-652.1973
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发表时间：
1973-01-01
影响因子：
5.4
通讯作者：
LEWIS, AM
中科院分区：
文献类型：
--
作者：
KELLY, TJ;LEWIS, AM
已分离出一系列腺病毒 2 (Ad2) 和猿猴病毒 40 (SV40)（无缺陷的 Ad2-SV40 杂合体）之间的可行重组体。该系列的成员（指定为 Ad2+ND1 至 Ad2+ND5）在早期 SV40 特异性抗原和它们在感染细胞中诱导的 SV40 特异性 RNA 种类方面彼此不同。 RNA-DNA 杂交技术显示，它们还含有不同量的 SV40 DNA。我们使用电子显微镜异源双链图谱技术检查了这些杂交体的 DNA 分子的结构。发现每个杂交体均含有特征大小的 SV40 DNA 单片段，共价插入到腺病毒 2 DNA 分子的独特位置。各种杂交体的 SV40 片段形成具有共同终点的重叠系列。当电子显微镜研究的结果与抗原诱导的数据相结合时，发现可以构建一个自洽的图谱，将SV40基因组的特定区域与特定抗原的诱导相关联。无缺陷杂交体中包含的SV40 DNA片段中这些早期SV40抗原诱导区的顺序是：U抗原、肿瘤特异性移植抗原和T抗原，其中U抗原区最接近共同终点。
A series of viable recombinants between adenovirus 2 (Ad2) and simian virus 40 (SV40) (nondefective Ad2-SV40 hybrids) have been isolated. The members of this series (designated Ad2+ND1through Ad2+ND5) differ from one another in the early SV40-specific antigens and the SV40-specific RNA species which they induce in infected cells. They also contain different amounts of SV40 DNA as shown by RNA-DNA hybridization techniques. We have examined the structure of the DNA molecules from these hybrids, using electron microscope heteroduplex mapping techniques. Each hybrid was found to contain a single segment of SV40 DNA of characteristic size covalently inserted at a unique location in the adenovirus 2 DNA molecule. The SV40 segments of the various hybrids formed an overlapping series with a common end point. When the results of the electron microscopic study were combined with data on antigen induction, it was found that a self-consistent map could be constructed which related specific regions of the SV40 genome to the induction of specific antigens. The order of these early SV40 antigen inducing regions in the SV40 DNA segments contained in the nondefective hybrids is: U antigen, tumor specific transplantation antigen, and T antigen with the U antigen region being nearest the common end point.