Effect of α-cyclodextrin-allyl isothiocyanate on ruminal microbial methane production in vitro
Effect of α-cyclodextrin-allyl isothiocyanate on ruminal microbial methane production in vitro
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α-环糊精-烯丙基异硫氰酸酯对瘤胃微生物体外产甲烷的影响
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发表时间：
2003
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通讯作者：
H. Itabashi
中科院分区：
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作者：
Z. A. Lila;N. Mohammed;S. Kanda;T. Kamada;H. Itabashi
本试验旨在研究α-环糊精-异硫氰酸烯丙酯（CD-AI）对瘤胃微生物甲烷产量和混合瘤胃微生物发酵玉米淀粉、马铃薯可溶性淀粉或干草加精料（1.5:1）的影响。将稀释后的瘤胃液（30 mL）在38℃厌氧孵育6和24 h，添加或不添加CD-AI（0、0.4、0.8、1.6和3.2 g/L）。CD-AI在所有底物中均未改变培养基的pH。乙酸摩尔比降低，丙酸摩尔比升高，乙酸与丙酸摩尔比相应降低（P < 0.05）。总挥发性脂肪酸和丁酸增加（P < 0.05）。氨氮含量降低（P < 0.05）。除马铃薯可溶性淀粉外，6 h后原生动物数量基本不变。CD-AI浓度从0增加到3.2 g/L时，玉米淀粉、马铃薯可溶性淀粉和干草加精料发酵的甲烷产量分别降低了49-100% （6 h）和14-100% (24 h) (P < 0.05)；39-100% （6 h）和16-100% (24 h)；45-100% （6 h）和17-100% （24 h）。以干草加精料为底物时，添加0.8 g/L CD-AI后6 h产甲烷菌数量显著减少（P < 0.05）。除低剂量（0.4 g/L） CD-AI外，24 h后干草加精料中性洗涤纤维消化率显著降低（P < 0.05）。因此，在不影响纤维消化的情况下，适当添加CD-AI可抑制甲烷产生，改善瘤胃发酵。
The objective of the present study was to investigate the effects of α-cyclodextrin-allyl isothiocyanate (CD-AI) on ruminal microbial methane production and rumen fermentation of corn starch, soluble potato starch or hay plus concentrate (1.5:1) by mixed rumen microorganisms. Diluted rumen fluid (30 mL) was incubated anaerobically at 38°C for 6 and 24 h with or without CD-AI (0, 0.4, 0.8, 1.6 and 3.2 g/L). The pH of the medium was unchanged by CD-AI in all substrates. The molar proportion of acetate was decreased and propionate was increased with a corresponding decrease in acetate : propionate ratio (P < 0.05). Total volatile fatty acids and butyrate were increased (P < 0.05). Ammonia-N was decreased (P < 0.05). Except with soluble potato starch, numbers of protozoa were unchanged after 6 h. As concentration of CD-AI increased from 0 to 3.2 g/L, fermentation of corn starch, soluble potato starch and hay plus concentrate resulted in decreased (P < 0.05) methane production of 49–100% (6 h) and 14–100% (24 h); 39–100% (6 h) and 16–100% (24 h); and 45–100% (6 h) and 17–100% (24 h), respectively. When hay plus concentrate was used as substrate, methanogenic bacteria were decreased (P < 0.05) with 0.8 g/L of CD-AI after 6 h. Excluding the lower dose level (0.4 g/L) of CD-AI, digestibility of neutral detergent fiber of hay plus concentrate was decreased (P < 0.05) after 24 h. A suitable level of CD-AI could therefore be used as a supplement to inhibit methane production and improve rumen fermentation without detrimental effects on fiber digestion.