Time-resolved detection of association/dissociation reactions and conformation changes in photosensor proteins for application in optogenetics
Time-resolved detection of association/dissociation reactions and conformation changes in photosensor proteins for application in optogenetics
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光传感器蛋白中缔合/解离反应和构象变化的时间分辨检测在光遗传学中的应用
DOI：
10.1007/s12551-021-00868-9
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
M. Terazima
中科院分区：
文献类型：
--
作者：
Noji Junpei;Yoshimori Akira;Ohnuki Jun;Takano Mitsunori;M. Terazima
光传感器蛋白的重要性不仅是因为它们的生物学功能，还因为它们在光遗传学中的应用。为了了解它们生物学功能背后的分子机制，从而寻求可能的光遗传学应用，需要阐明它们在光激发下分子间相互作用的动力学(例如，缔合/解离反应和构象变化)。虽然用传统的光谱技术很难在时间域中跟踪这种反应，但基于瞬时光栅技术的时间分辨扩散方法已经被证明在以时间分辨的方式检测这种光谱沉默的动力学方面具有显著的优势。本文介绍了蓝光感受器蛋白--趋光素的原理及研究进展。测定了二聚反应、解离反应和构象变化的反应动力学，确定了反应方案。该方法可以用来从扩散的角度研究蛋白质的反应，并阐明其他光谱方法无法检测到的反应方案和动力学。
Photosensor proteins are important not only because of their biological functions but also because of their applications in optogenetics. To understand the molecular mechanism behind their biological functions and consequently seek possible applications to optogenetics, the dynamics of their intermolecular interaction (for example, association/dissociation reaction and conformational changes) upon photoexcitation need to be elucidated. Although it has been difficult to trace such reactions in the time domain using traditional spectroscopic techniques, the time-resolved diffusion method based on the transient grating technique has been demonstrated to possess a significant advantage in detecting such spectrally silent dynamics in a time-resolved manner. In this paper, the principle and studies on blue light sensor proteins, phototropins, are described. Reaction kinetics of dimerization, dissociation reactions, and conformational changes were measured, and reaction schemes were determined. This method can be employed to study protein reactions from the viewpoint of diffusion and to elucidate the reaction schemes and kinetics that cannot be detected by other spectroscopic methods.