INTERFERON-STIMULATED TRANSCRIPTION - ISOLATION OF AN INDUCIBLE GENE AND IDENTIFICATION OF ITS REGULATORY REGION
INTERFERON-STIMULATED TRANSCRIPTION - ISOLATION OF AN INDUCIBLE GENE AND IDENTIFICATION OF ITS REGULATORY REGION
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DOI：
10.1073/pnas.83.23.8929
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发表时间：
1986-12-01
影响因子：
11.1
通讯作者：
DARNELL, JE
中科院分区：
文献类型：
--
作者：
LEVY, D;LARNER, A;DARNELL, JE
一种人类基因（称为ISG-54K），可被. α诱导从几乎不可检测的水平到高转录活性。-和。beta。-干扰素已被克隆。测定了编码区的基因组结构和核苷酸序列，确定了RNA起始位点。在重组质粒和腺病毒载体中，将该基因的5‘’部分与缺乏活性启动子的异源基因融合。这些融合基因被用来评估ISG-54K启动子对干扰素的反应活性。重组质粒仅在干扰素作用下才能在HeLa细胞中形成RNA。此外，在人二倍体成纤维细胞中，重组腺病毒载体感染导致特异性RNA响应干扰素增加50倍，随后减少，模仿内源基因的自然调节转录周期。
A human gene (termed ISG-54K) that is induced from near undetectable levels to high transcriptional activity by .alpha.- and .beta.-interferons has been cloned. The genomic structure and nucleotide sequence of the coding region were determined and the RNA initiation site was identified. The 5'' portion of the gene was fused with a heterologous gene lacking an active promoter in recombinant plasmid and adenoviral vectors. These fusion genes were used to assess the activity of the ISG-54K promoter in response to interferon. RNA was formed in HeLa cells from recombinant plasmids only in response to interferon. Furthermore, in human diploid fibroblasts, infection with the recombinant adenovirus vector resulted in a 50-fold increase in specific RNA in response to interferon, followed by a subsequent decrease, imitating the natural regulated transcriptional cycle of the endogenous gene.