Enhanced Release of Chitosanase from Streptomyces griseus through Direct Interaction of Liposome with Cell Membrane under Heat Stress
Enhanced Release of Chitosanase from Streptomyces griseus through Direct Interaction of Liposome with Cell Membrane under Heat Stress
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DOI：
10.1263/jbb.106.602
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发表时间：
2008-12-01
影响因子：
2.8
通讯作者：
Kuboi, Ryoichi
中科院分区：
文献类型：
--
作者：
Ngo, Kien Xuan;Umakoshi, Hiroshi;Kuboi, Ryoichi
在41℃热应激条件下，1-棕榈酰-2-油酰- n-甘油-3-磷脂胆碱脂质体与灰链霉菌细胞膜的直接相互作用使壳聚糖酶的产量和释放量比37℃无POPC脂质体时提高了2.2倍。在41℃的高温下，在有POPC脂质体存在的情况下，壳聚糖酶通过脂质模拟细胞膜（LMCM）脂质体释放的量是初始捕获壳聚糖酶的17%，而在没有POPC脂质体的情况下，即使在41℃的热应激下，壳聚糖酶的释放量也只有1%，清楚地显示了膜与膜之间直接相互作用的重要性。
A direct interaction of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) liposomes with membrane of Streptomyces griseus cell under the heat stress at 41 degrees C increased the chitosanase production and release to 2.2 times higher than that at 37 degrees C without the POPC liposomes. Amount of chitosanase released across the lipid mimicking cell membrane (LMCM) liposome under a heat at 41 degrees C in the presence of POPC liposomes was 17% of initially-entrapped chitosanase while it was only 1% in the absence of POPC liposomes, even under a heat stress at 41 degrees C, clearly showing the importance of the direct interaction between membrane and membrane.