Reexpression of glial fibrillary acidic protein rescues the ability of astrocytoma cells to form processes in response to neurons.
Reexpression of glial fibrillary acidic protein rescues the ability of astrocytoma cells to form processes in response to neurons.
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DOI：
10.1083/jcb.127.3.813
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发表时间：
1994-11
期刊：
影响因子：
--
通讯作者：
Liem RK
中科院分区：
文献类型：
--
作者：
Chen WJ;Liem RK
在中枢神经系统发育过程中，星形胶质细胞在协调神经元的迁移和定位中起着重要作用。当与颗粒神经元共培养时，原代星形胶质细胞以及星形细胞瘤细胞将延长长期稳定的过程。为了确定胶质细胞酸性蛋白（GFAP）的功能，在星形胶质细胞和星形细胞瘤细胞中的主要中间丝蛋白，我们通过稳定转染人星形细胞瘤U251 MG细胞中的反义GFAP构建体来抑制GFAP的表达。所得到的AS 2-U251细胞在颗粒神经元的存在下不能再延长稳定的过程。为了证明这种效应是由于GFAP的缺乏，我们将完全编码的大鼠脑GFAP cDNA重新引入这些AS 2-U251细胞中。由此产生的大鼠GFAP表现为丝状网络，并且GFAP的再表达挽救了这些星形细胞瘤细胞在与神经元共培养时形成稳定过程的能力。从这些结果中，很明显，胶质细胞特异性中间丝蛋白，GFAP，需要这些星形细胞瘤细胞的过程中响应颗粒神经元的延伸。
Astroglial cells play an important role in orchestrating the migration and positioning of neurons during central nervous system development. Primary astroglia, as well as astrocytoma cells will extend long stable processes when co-cultured with granule neurons. In order to determine the function of the glial fibrillary acidic protein (GFAP), the major intermediate filament protein in astroglia and astrocytoma cells, we suppressed the expression of GFAP by stable transfection of an anti- sense GFAP construct in human astrocytoma U251MG cells. The resulting AS2-U251 cells can no longer extend stable processes in the presence of granule neurons. To show that this effect is due specifically to the absence of GFAP, we reintroduced a fully encoding rat brain GFAP cDNA into these AS2-U251 cells. The resulting rat GFAP appeared as a filamentous network and the reexpression of GFAP rescued the ability of these astrocytoma cells to form stable processes when co-cultured with neurons. From these results, it is clear that the glial specific intermediate filament protein, GFAP, is required for process extension of these astrocytoma cells in response to granule neurons.