BM-40 (Osteonectin, SPARC) is expressed both in the epidermal and in the dermal compartment of adult human skin
BM-40 (Osteonectin, SPARC) is expressed both in the epidermal and in the dermal compartment of adult human skin
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DOI：
10.1046/j.1523-1747.1998.00094.x
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发表时间：
1998-02-01
影响因子：
6.5
通讯作者：
Nischt, R
中科院分区：
文献类型：
--
作者：
Hunzelmann, N;Hafner, M;Nischt, R
BM-40（Osteonectin，骨粘连蛋白）是人成骨细胞分泌的最丰富的糖蛋白。对小鼠组织中BM-40 mRNA表达的杂交研究已经证明是最高的。在骨中的转录水平，但表达也观察到在其他几个间充质组织。相反，关于BM-40在人体组织中的表达，特别是原位表达，我们知之甚少。通过北方印迹分析从正常人皮肤获得的总RNA，并揭示BM-40的显著表达。为了分析其在体内的表达，进行原位杂交，证明BM-40在真皮中的成纤维细胞、平滑肌和内皮细胞中表达。有趣的是，BM-40 mRNA也在整个基底、棘突、和成人皮肤表皮中的颗粒层。进一步免疫组化分析显示，BM-40蛋白在真皮中的沉积最强烈，在真皮乳头层的基底膜正下方和血管以及腺体结构周围。在表皮中，BM-40蛋白可在基底层上的细胞间检测到。这项研究表明，BM-40，以前被认为是专门在细胞外基质产生细胞中表达，实际上可能在表皮的分化和维护中发挥作用。
BM-40 (Osteonectin, SPARC) is the most abundant glycoprotein secreted by human osteoblasts. hybridization studies on the expression of BM-40 mRNA in murine tissues have demonstrated the highest. levels of transcripts in bone, but expression was also observed in several other mesenchymal tissues. In contrast, little is known about the expression of BM-40 in human tissues, especially in situ. Total RNA obtained from normal human skin was analyzed by northern blotting and revealed a marked expression of BM-40, To analyze its expression in vivo, in situ hybridization was performed, demonstrating that BM-40 is expressed in fibroblasts, smooth muscle, and endothelial cells in the dermis, Interestingly, BM-40 mRNA was also detected throughout the basal, spinous, and granular layers in the epidermis of adult human skin. Further analysis by immunohistochemistry revealed a marked deposition in the dermis that was most intense-directly below the basement membrane in the papillary dermis and around vascular as well as glandular structures, In the epidermis, BM-40 protein could be detected intercellularly in suprabasal layers, This finding is further supported by the intercellular deposition of BM40 detected by immunofluorescence in cultured keratinocytes. This study demonstrates that BM-40 that has previously been thought to be exclusively expressed in extracellular matrix producing cells may in fact play a role in differentiation and maintenance of the epidermis.