Mycoviruses mediate mycotoxin regulation in Aspergillus ochraceus
Mycoviruses mediate mycotoxin regulation in Aspergillus ochraceus
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DOI：
10.1111/1462-2920.14436
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发表时间：
2019-06-01
影响因子：
5.1
通讯作者：
Turina, M.
中科院分区：
文献类型：
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作者：
Nerva, L.;Chitarra, W.;Turina, M.
迄今为止，尚无证据表明，在真菌病毒的存在与真菌毒素的定量或定性调节之间存在直接关联。在我们的研究中，我们用一种不同的赭曲霉分离物和金黄色青霉分离物纯化的分枝病毒转染了无病毒的赭曲霉产赭曲霉毒素a （OTA）分离物。在测试的分支病毒中，只有牛曲霉病毒（AoV）是在牛曲霉中广泛存在的一种局部病毒，它引起了一种特定的相互作用，导致OTA的过量生产，OTA受欧盟委员会监管，是食品和饲料商品的第二大污染物。基因表达分析未能揭示在OTA生物合成途径中被认为发挥作用的基因mRNA的特异性病毒上调。此外，AoOTApks1，一个被认为是OTA生产所必需的聚酮合成酶基因，在我们的OTA生产分离物的基因组中令人惊讶地缺失。可能的生物学和进化意义的分枝病毒调控真菌毒素的生产进行了讨论。
To date, no demonstration of a direct correlation between the presence of mycoviruses and the quantitative or qualitative modulation of mycotoxins has been shown. In our study, we transfected a virus-free ochratoxin A (OTA)-producing isolate of Aspergillus ochraceus with purified mycoviruses from a different A. ochraceus isolate and from Penicillium aurantiogriseum. Among the mycoviruses tested, only Aspergillus ochraceus virus (AoV), a partitivirus widespread in A. ochraceus, caused a specific interaction that led to an overproduction of OTA, which is regulated by the European Commission and is the second most important contaminant of food and feed commodities. Gene expression analysis failed to reveal a specific viral upregulation of the mRNA of genes considered to play a role in the OTA biosynthetic pathway. Furthermore, AoOTApks1, a polyketide synthase gene considered essential for OTA production, is surprisingly absent in the genome of our OTA-producing isolate. The possible biological and evolutionary implications of the mycoviral regulation of mycotoxin production are discussed.